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|Table of Contents|

雪落樱再生体系的建立(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2014年增刊
Page:
20-24
Column:
研究论文
publishdate:
2014-10-29

Article Info:/Info

Title:
Establishment of regeneration system in Cerasus xueluoensis
Article ID:
1000-2006(2014)S1-0020-05
Author(s):
SHI Gangying NAN Chenghui YI Xiangui ZHANG Kaiwen WANG Xianrong*
College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China
Keywords:
Cerasus xueluoensis explant plant regeneration tissue culture
Classification number :
S503.53
DOI:
10.3969/j.issn.1000-2006.2014.S1.005
Document Code:
A
Abstract:
A plant regeneration system via adventitious shoot formation from leaf explants of Cerasus xueluoensis was established. Several factors such as explant sterilizing time, hormone concentration and concentration of sucrose and so on were explored to optimize the regeneration system in virto. The result showed that the fully unfold leaves were selected as the most appropriate explant in spring. And the best basic medium is MS. With 6-BA(1.0 mg/L)+NAA(0.5 mg/L)+Vc(20 mg/L)was the optimum medium for callus proliferation. And 20 mg/L Vc was best to inhibit C. xueluoensis callus from browning. MS+6-BA(2.0 mg/L)+NAA(0.1 mg/L)+TDZ(2.0 mg/L)+Vc(20 mg/L)was the optimum medium for shoot differentiation. And 3/4MS+NAA(0.1 mg/L)+sucrose(15 g/L)was the optimum medium for root differentiation. The rooting percentage was 100% after 27 days.

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