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植物胚拯救技术的影响因素及其应用(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2014年05期
Page:
143-148
Column:
综合述评
publishdate:
2014-09-30

Article Info:/Info

Title:
Influence factors and application of plant embryo rescue techniques
Article ID:
1000-2006(2014)05-0143-06
Author(s):
ZHANG Qianyun1 WANG Ying2 DU Yuliang1 YIN Zengfang1*
1.College of Biologica and Environment, Nanjing Forestry University, Nanjing 210037, China;
2.Jiangsu Provincial Agricultural Sciences Institute of Along the Yangtze River, Nantong 226001, China
Keywords:
embryo rescue techniques plant breeding embryo culture
Classification number :
Q944
DOI:
10.3969/j.issn.1000-2006.2014.05.028
Document Code:
A
Abstract:
Embryo rescue techniques play an important role in saving abortive embryos, preventing embryo degeneration at early development stage and developing new triploid and polyploid. There were three main methods of plant embryo rescue: in vivo embryo transfer, embryo in vivo culture and embryo in vitro culture. In the process of plant embryo in vitro culture,according to the development degree of the plant embryo which was needed to save, three training modes were set up,that is, in vitro ovary culture, ovule culture and immature embryo culture. A plenty of researches have been shown that embryo age, medium and medium phase, culture conditions, osmotic pressure, growth regulator, complementary materials, genotype and so on, played a great role in the success of embryo rescue. Therefore, embryo rescue techniques as an adjunct to conventional breeding methods will have further room for improvement in such aspects as embryonic age limit and plant regeneration rate. In addition, combining embryo rescue techniques with molecular biological research methods, genetic transformation is expected to stabilize different characteristics of the new germplasm, and innovate the plant resources.

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Last Update: 2014-10-31