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普通油茶体胚再生体系研究(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2014年06期
Page:
160-164
Column:
研究简报
publishdate:
2014-12-09

Article Info:/Info

Title:
Study on somatic embryogenesis regeneration system in Camellia oleifera
Article ID:
1000-2006(2014)06-0160-05
Author(s):
HU Yuling YAO Xiaohua* REN Huadong WANG Kailiang LONG Wei
Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, China
Keywords:
Camellia oleifera Abel somatic embryogenesis regeneration plant hormones
Classification number :
S722
DOI:
10.3969/j.issn.1000-2006.2014.06.030
Document Code:
A
Abstract:
In order to establish genetic transformation receptor system for oil camellia plant via agrobacterium-mediated, ‘Changlin' 4,53 and 166 immature zygotic embryos were used as explants to carry out multi-level and multi-factor tissue culture experiments,the results showed that: the effect of culture medium pH on the callus induction rate was not significant, but varieties had a significant effect on callus induction rate. Somatic embryo regeneration was observed from ‘Changlin' 53. MS and 2,4-D and TDZ were suitable for the embryo regeneration, the somatic embryogenesis induction rate was 98%, using MS+2 mg/L 2,4-D+0.5 mg/L TDZ, the somatic embryo induction rate was 89.6% based on MS+1 mg/L 2,4-D+2 mg/L KT+500 mg/L CH, and in addition 2,4-D, KT, TDZ and additives experiments, the somatic embryogenesis induction rate of ‘Changlin' 53 was 100%. In hormone-free MS medium, the differentiation embryos could regenerate to plant.

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Last Update: 2014-12-31