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抗松材线虫病赤松胚性愈伤组织的诱导及增殖(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2015年01期
Page:
17-21
Column:
专题报道
publishdate:
2015-01-30

Article Info:/Info

Title:
Induction and proliferation of embryogenic callus in nematode-resistant Pinus densiflora
Article ID:
1000-2006(2015)01-0017-05
Author(s):
WU Jing ZHU LihuaXU Jianxiu WU Xiaoqin YE Jianren*
Collaborative Innovation Center of Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China
Keywords:
nematode-resistant Pinus densiflora immature zygotic embryo embryogenic callus DCR medium
Classification number :
S763; Q813
DOI:
10.3969/j.issn.1000-2006.2015.01.004
Document Code:
A
Abstract:
Embryogenic callus was induced from immature embryos of nematode-resistant Pinus densiflora. Factors affecting callus initiation were investigated. The results showed that successful initiation of embryogenic callus depended heavily on embryo developmental stage, the combination of auxin and cytokinins, and carbon source. The initiation of embryogenic callus worked best when immature mega gametophytes developed at stage 2-4 were used as starting material. The optimal combination of hormone was 2 mg /L 2,4-D and 1 mg /L 6-BA with the induction rate of 5%. Maltose was better than sucrose and glucose. Further proliferation of embryogenic callus was achieved by transfering explants to a medium with reduced levels of plant growth regulators.

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Last Update: 2015-01-31