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培养基种类及激素配方对青钱柳悬浮细胞次生代谢物质积累的影响(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2015年02期
Page:
47-51
Column:
研究论文
publishdate:
2015-04-01

Article Info:/Info

Title:
Influence of types of basic medium, hormone formulations on accumulation of secondary metabolites in Cyclocarya paliurus suspension cells
Article ID:
1000-2006(2015)02-0047-05
Author(s):
NGUYEN ThiXuyen123YANG Wanxia12FANG Shengzuo12*CAO Yanni12
1. Co-Inmovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China;
2. College of Forestry,Nanjing Forestry University,Nanjing 210037,China;
3. College of Forestry,Vietnam Bac Giang Agriculture-Forestry University,Bac Giang 236271,Vietnam
Keywords:
Cyclocarya paliurus suspension cells total flavonoid total triterpenoid total polysaccharides
Classification number :
S792.12
DOI:
10.3969/j.issn.1000-2006.2015.02.008
Document Code:
A
Abstract:
Cyclocarya paliurus callus induced with its aseptic seedling were cultured in suspension culture. The types of basic medium, hormone formulations were studied to select the suitable Cyclocarya paliurus production of secondary metabolites in plant cell suspension culture system. The results were shown as follows: The best medium of Cyclocarya paliurus suspension cell culture medium was MS, hormone formulations was 1.0 mg/L NAA+0.5 mg/L KT. The basic medium was MS which could increase the suspension cell dry weight and the production of secondary metabolites in the medium.B1(1.0 mg/L NAA+0.5 mg/L KT)hormone formulations was propitious to flavonoids accumulation in cell suspension.B3(1.0 mg/L IBA+0.5 mg/L TDZ)hormone formulations was propitious to polysaccharides accumulation in cell suspension.B2(0.5 mg/L 2,4-D+0.3 mg/L NAA)hormone formulations was propitious to triterpenoid accumulation in cell suspension. Types of basic medium, hormone formulations on accumulation of secondary metabolites in Cyclocarya paliurus suspension cells were preliminarily selected. The results laid the foundation for the Cyclocarya paliurus suspension cell culture system.

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Last Update: 2015-03-31