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柳杉优良无性系离体培养再生体系研究(PDF/HTML)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2015年03期
Page:
55-58
Column:
研究论文
publishdate:
2015-05-30

Article Info:/Info

Title:
Study on regeneration system of Cryptomeria clones in vitro culture
Article ID:
1000-2006(2015)03-0055-04
Author(s):
ZHU Chenchen12 XU Jin12* OUYANG Lei3 SHI Jisen12
1.College of Forestry, Nanjing Forestry University, Nanjing 210037, China;
2.Co-Innovation Cnter for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China;
3. Fujian Academy of Forestry, Fuzhou 350012, China
Keywords:
Cryptomeria clones plant regeneration
Classification number :
S722
DOI:
10.3969/j.issn.1000-2006.2015.03.011
Document Code:
A
Abstract:
The regeneration system in this paper was established by using soft stem of Cryptomeria as explants. Using orthogonal experimental design, among which six Cryptomeria clones were used for the bud proliferation and three clones were used for rooting induction. Main conclusions were shown as follows:① Adventitious bud could be induced by six different clones on DCR basic medium supplement with different combinations of 6-BA and NAA. But there are differences in bud proliferation ability in the different clones. The bud proliferation coefficient was up to 5.00(Jing 1#).② During the process of rooting culture of Cryptomeria clones, 1/2DCR medium was the optimum basic culture medium. And the rooting rate of three clones which had been experimented all reached the highest when added 0.10 mg/L IBA and 0.20 mg/L NAA. Among them, the rooting rate of 011# clone was up to 93.33%. The research will provide technical support for shortening the breeding cycle of the clones of Cryptomeria.

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Last Update: 2015-05-30