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板栗愈伤组织对栗疫菌Cp-毒素的抗性响应(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2015年05期
Page:
1-6
Column:
专题报道
publishdate:
2015-10-20

Article Info:/Info

Title:
Resistance responses of Castanea mollissima callus to Cp-toxin under Cryphonectria parasitica stress
Article ID:
1000-2006(2015)05-0001-06
Author(s):
HAN Shan12 ZHU Tianhui12* QIAO Tianmin3 LI Shujiang12 WANG Ju2 ZHANG Boyang2
1. College of Forestry,Sichuan Agricultural University,Ya'an 625014,China;
2. Sichuan Provincial Key Laboratory of Forest Protection,Ya'an 625014,China;
3. Sichuan Provincial Key Laboratory of Ecological Forestry Engineering for Upper Reach of Changjiang River,Ya'an 625014,China
Keywords:
Cryphonectria parasitica Chinese chestnut callus Cp-toxin pathogenesis-related protein resistance substance
Classification number :
S763
DOI:
10.3969/j.issn.1000-2006.2015.05.001
Document Code:
A
Abstract:
In order to clarify the resistance mechanism of different chestnut cultivars to Cp-toxin stress, effects of different concentrations of Cp-toxin from Cryphonectria parasitica on the activities of chitinase, β-1,3-glucanase, the contents of phytoalexin, free proline(Pro)of chestnut callus were analyzed.In this study, a significant correlation was observed between the treatment concentration and chestnut resistance. The results showed that the activities of enzymes increased notably when treated with the low concentrations of Cp-toxin(5-100 μg/mL). When treated with the high concentrations of Cp-toxin(>100 μg/mL),the activities of enzymes and the contents of phytoalexin decreased notably.These data suggested that resistant materials in different varieties increased significantly under the treatment with low concentration of Cp-toxin. This study also indicates that the chitinase, β-1,3-glucanase and phytoalexins were the inherent mechanism for resistance of chestnut callus to toxin stress, and change of free proline content was not an important factor related to the resistance.

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Last Update: 2015-10-15