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DNA条形码试剂盒检测技术在墨天牛鉴定中的应用(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2015年06期
Page:
7-11
Column:
专题报道
publishdate:
2015-11-30

Article Info:/Info

Title:
The application of DNA barcoding identification kit technology to Monochamus Dejean
Article ID:
1000-2006(2015)06-0007-05
Author(s):
CHEN Mengyi12 XU Mei3 QIAN Lu3 AN Yulin3 HAO Dejun12*
1. Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China;
2. College of Forestry, Nanjing Forestry University, Nanjing 210037, China;
3. Jiangsu Entry-exit Inspection and Quarantine Bureau, Nanjing 210001, China
Keywords:
Monochamus COⅠ gene DNA barcoding identification kit technology specific site quarantine
Classification number :
S763
DOI:
10.3969/j.issn.1000-2006.2015.06.002
Document Code:
A
Abstract:
In order to rapidly and accurately identify the Monochamus of wood from Russian, we employed DNA barcode detection kit technology and designed a pair of nested primers based on mitochondrial COⅠ gene(mitochondrial cytochrome oxidase subunit I). Furthermore, COⅠ gene sequence of eight Monocharnus species was amplificated, and uploaded to pest information site. According to differences of arrangement and composition among these species at base sites, species unique bases was got in the corresponding sites, which is named specific site to distinguish this species. In this way, we developed the DNA barcoding identification kit technology to Monochamus species, which will provide a new way for port quarantine identification.

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Last Update: 2015-11-30