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NaCl处理对银杏悬浮培养细胞生长、 耐盐性和黄酮积累的影响(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2015年06期
Page:
45-50
Column:
研究论文
publishdate:
2015-11-30

Article Info:/Info

Title:
Effects of NaCl treatment on the growth, salt tolerance and flavonoids accumulation in Ginkgo biloba suspension cells
Article ID:
1000-2006(2015)06-0045-06
Author(s):
CHEN Ying12 LUO Yongya12 QIU Nafei12 WANG Ruiqi12 SHENG Lili12 CAO Fuliang13
1.Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University,Nanjing 210037, China;
2.College of Biology and Environment,Nanjing Forestry University,Nanjing 210037,China;
3. College of Forestry,Nanjing Forestry University,Nanjing 210037,China
Keywords:
Ginkgo biloba L. suspension cells salt tolerance flavonoids accumulation
Classification number :
S723
DOI:
10.3969/j.issn.1000-2006.2015.06.009
Document Code:
A
Abstract:
The salt tolerance ability, cell growth and flavonoids accumulation in Ginkgo biloba L. suspension cells were investigated by adding 0, 50, 100, 150 mmol/L NaCl into the MS liquid culture medium. The results showed that the cell growth increased with the increasing of both salt treatment time and concentration in the early treatment(4-8 days), while decreased during 12-20 days under NaCl treatments. Moderate and high dose of salinity(100-150 mmol/L)had a certain tolerance to salt stimulation by increasing the soluble protein content at early NaCl treatment(4-8 days)compared to the control. The growth biomass was reduced by the increase of salinity dose during 8-16 days, whereas the high level of proline and flavonoids was found and it suggested that the Ginkgo biloba cells could enhance the osmotic adjustment capacity and oxidation resistance ability by stimulating the biosynthesis of proline and flavonoids at this period. Furthermore, the intensity of salt stress was higher than the cell self-regulation capacity, and serious damage and brown appeared in cells at the 20th day. The growth of suspension cells was not obviously restrained under 50 mmol/L NaCl treatment compared to the control, due to the low level of relative electric conductivity(REC)and MDA content in cell observed. Furthermore, the PAL activity increased significantly at the 8th day and the highest flavonoid accumulation and total flavonoids yields(129.0 mg/L)were found at 12th day. The flavonoids content at 100 mmol/L NaCl treatment increased by 29.0% than the control, while the total flavonoids yield(98.4 mg/ L)was only 10.9% higher than the control. However, the flavonoids content at 150 mmol/L NaCl could maintain the high level at 8th day and it was 66.3% times higher than control, meanwhile the total flavonoids yield was(104.0 mg /L)attained at 8th day, too. Therefore, the cell sampled time at 12th day and at 8th day was beneficial to flavonoids production under 50 mmol/L and 150 mmol/L NaCl treatment, respectively.

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Last Update: 2015-11-30