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重组血红密孔菌漆酶在染料脱色中的应用(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2016年01期
Page:
92-96
Column:
研究论文
publishdate:
2016-01-31

Article Info:/Info

Title:
Application of recombinant Pycnoporus sanguineus laccase in dye decolorization
Article ID:
1000-2006(2016)01-0092-05
Author(s):
WANG TiannüLI GuofuZHAO Min*LI JunLU Lei
College of Life Science,Northeast Forestry University, Harbin 150040, China
Keywords:
recombinant laccase mediator dye decolorization waste water treatment Pycnoporus sanguineus
Classification number :
X172
DOI:
10.3969/j.issn.1000-2006.2016.01.015
Document Code:
A
Abstract:
With the aim of investigating the dye decolorization ability of recombinant Pycnoporus sanguineus laccase expressed by Pichia pastoris, we performed the decolorization of three typical synthetic dyes,emazol brilliant blue R(RBBR),reactive black 5(RB5)and indigo carmine(IC),by recombinant laccase using single factor design. Furthermore, we studied the decolorization of mixed dyes composed by these three aforementioned dyes by recombinant laccase. The results indicated that recombinant laccase was weak in dye decolorization without mediation of mediator. Syringaldehyde was the optimum mediator for the recombinant laccase to degrade three synthetic dyes. The optimal pH and temperature for laccasemediator system decolorization were 5.0 and 50 ℃. Under optimal condition, the decolorization efficiency of mixed dyes reached 93%, which demonstrates the potential application of the recombinant laccase in wastewater treatment.

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Last Update: 2016-02-25