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不同活性炭对杂交鹅掌楸体胚发生的影响(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2016年02期
Page:
59-64
Column:
研究论文
publishdate:
2016-03-30

Article Info:/Info

Title:
Effects of different activated carbon on somatic embryogenesis of Liriodendron hybrids
Article ID:
1000-2006(2016)02-0059-06
Author(s):
LU Lu LU Ye SHENG Yu ZHENG Chen SHI Jisen CHEN Jinhui*
Co-Innovation Center for the Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing 210037, China
Keywords:
Liriodendron hybrids somatic embryogenesis activated carbon
Classification number :
S718
DOI:
10.3969/j.issn.1000-2006.2016.02.010
Document Code:
A
Abstract:
Activated carbon is often used to improve cell growth and development in somatic embryogenesis. In this study, three types of activated carbon, including phosphoric acid activated carbon, activated carbon for refinement of injection and activated carbon purchased from Sigma were added respectively to the mediums for improving somatic embryogenesis frequency of two genotypes of cell lines in Liriodendron hybrids. The number of total somatic embryos and cotyledon embryos were counted, pH and osmotic pressure of the mediums were detected, and the adsorption ability of activated carbon were analyzed to select the most suitable activated carbonl and to find their potential effects on somatic embryogenesis of Liriodendron hybrids. The results showed that the first two types of activated carbon induced more total somatic embryos and cotyledon embryos than the third type, especially the activated carbon for refinement of injection. The adsorption ability of activated carbon for refinement of injection was moderate among the three, and the pH and osmotic pressure of the mediums containing it were also temperate compared with the other two types of activated carbon. It was concluded from the results that the adsorption ability of activated carbon may change the pH and osmotic pressure of mediums directly to influence the somatic embryogenesis of Liriodendron hybrids.

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Last Update: 2016-04-01