我们的网站为什么显示成这样?

可能因为您的浏览器不支持样式,您可以更新您的浏览器到最新版本,以获取对此功能的支持,访问下面的网站,获取关于浏览器的信息:

|Table of Contents|

光强对喜树幼苗生长及喜树碱含量的影响(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2016年03期
Page:
9-14
Column:
专题报道
publishdate:
2016-05-18

Article Info:/Info

Title:
Effects of light intensity on the growth of Camptotheca acuminata seedlings and camptothecin contents
Article ID:
1000-2006(2016)03-0009-06
Author(s):
ZHOU Xin1 WANG Guibin1* LIU Lin1 CAO Fuliang1 ZU Yuangang2
1. Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China;
2. Key Laboratory of Forest Plant Ecology of Ministry of Education, College of Forestry, Northeast Forestry University, Harbin 150040, China
Keywords:
Camptotheca acuminata light intensity growth of seedling primary metabolite camptothecin
Classification number :
S718; S792.99
DOI:
10.3969/j.issn.1000-2006.2016.03.002
Document Code:
A
Abstract:
Camptotheca acuminata, a rapid growing tree indigenous to south China, which can produce a kind of anticancer secondary metabolite, camptothecin(CPT). CPT is classified as a modified monoterpenoidindole alkaloid that has attracted great attention for its significant role in antitumor activity. It is presumed that CPT is one of the defence compounds of C.acuminata, but the contents of CPT are usually very low. Previous reports have shown that environmental factors such as light intensity can affect plant growth and metabolite synthesis. To enhance CPT yield in C. acuminata, we observed the effects of light intensity on CPT production in the leaves of C. acuminata. We use three levels of light intensity, namely L1 300, L2 500, L3 800 μmol/(m2·s). The samples were collected from the third to fifth leave from the seedling top at the 7, 14, 21 and 28 days after treatment. The results were shown as follows. At L1, there were great increments in the seedling height and caliper. With the increase of light intensity, the root cap ratio increased in the beginning and then decreased, while the biomass generally showed a decreasing trend. The seedlings possessed the highest soluble sugar and soluble protein accumulation at L3. Both L1 and L3 had positive effect on TDC activities, and in the meantime, there was a great enhancement in CPT concentration and yield per plant at L1. This may have important implications for the production of the antitumor drug CPT in plantations at an industrial scale.

References

[1] 鲍思伟, 谈锋. 不同光强对曼地亚红豆杉生长及生理代谢的影响[J]. 防护林科技, 2009(2): 6-8, 34. Doi:10.3969/j.issn.1005-5215.2009.02.017. Bao S W, Tan F. Effect of different light intensities on growth and physiological metalolism of Taxus media[J]. Protection Forest Science and Technology, 2009(2):6-8, 34.
[2] 沈晓霞, 盛束军, 徐建中. 环境因子对益母草总生物碱含量的影响[J]. 浙江农业学报, 2002, 14(4): 221-225. Doi:10.3969/j.issn.1004-1524.2002.04.008. Shen X X, Sheng S J, Xu J Z. Effect of environmental factors on the total alkaloid content in Yimu Cao(Leonurus Artemisia S.Y. Hu)[J]. Acta Agriculturae Zhejiangensis, 2002, 14(4): 221-225.
[3] 唐中华, 郭晓瑞, 于景华, 等. 弱光对长春花(Catharanthus roseus)幼苗中可溶性糖、生物碱及激素含量的影响[J]. 生态学报, 2007, 27(11): 4419-4424. Doi:10.3321/j.issn:1000-0933.2007.11.006. Tang Z H, Guo X R, Yu J H, et al. Effects of low light intensity on changes of soluble sugars, alkaloids and phytohormones in Catharanthus roseus seedlings[J]. Acta Ecologica Sinica, 2007, 27(11): 4419-4424.
[4] Cai Z Q, Wang W H, Wang J, et al. Growth, photosynthesis and root reserpine concentrations of two Rauvolfia species in response to a light gradient[J]. Industrial Crops and Products, 2009, 30: 220-226. Doi:10.1016/j.indcrop.2009.03.010.
[5] 戴绍军, 王洋, 阎秀峰, 等. 滤光膜对喜树幼苗叶片生长和喜树碱含量的影响[J]. 生态学报, 2004, 24(5): 869-875. Doi:10.3321/j.issn:1000-0933.2004.05.001. Dai S J, Wang Y, Yan X F, et al. Effect of color films on growth and camptothecin content in the leaves of Camptotheca acuminata seedlings[J]. Acta Ecologica Sinica, 2004, 24(5): 869-875.
[6] 王博文, 王洋, 阎秀峰. 光强对喜树幼苗喜树碱含量及分配的影响[J]. 黑龙江大学自然科学学报, 2006, 23(2): 260-264. Doi:10.3969/j.issn.1001-7011.2006.02.030. Wang B W, Wang Y, Yan X F. Effect of light intensity on camptothecin content and allocation in Camptotheca acuminata seedlings[J]. Journal of Natural Science of Heilongjiang University, 2006, 23(2):260-264.
[7] Liu Y, Song L L, Yu W W, et al. Light quality modifies camptothecin production and gene expression of biosynthesis in Camptotheca acuminata Decne. seedlings[J]. Industrial Crops and Products, 2015,66: 137-143.
[8] 孙世芹, 阎秀峰. 氮素水平对喜树幼苗喜树碱含量的影响[J]. 中国中药杂志, 2008, 33(4):354-359. Doi:10.3321/j.issn:1001-5302.2008.04.003. Sun S Q, Yan X F. Effect of different nitrogen levels on camptotheca content of Camptotheca acuminata seedlings[J]. China Journal of Chinese Materia Medica, 2008,33(4):354-359.
[9] 应叶青, 刘鹏, 王兴华, 等. 干旱对不同种源喜树苗木生长及叶片喜树碱产量的影响[J]. 林业科学, 2012, 48(11):30-35. Doi:10.11707/j.1001-7488.20121105. Ying Y Q, Liu P, Wang X H, et al. Effect of drought stress on the seedling growth from different provenances and camptothecin yield in leaves of Camptotheca acuminata[J]. Scientia Silvae Sinicae, 2012, 48(11): 30-35.
[10] 刘永军, 郭守华, 杨晓玲. 植物生理生化实验[M]. 北京: 中国农业科技出版社, 2002:128-129.
[11] 张立军,樊金娟.植物生理学实验教程[M].北京:中国农业大学出版社,2007:57-72.
[12] 牧文. 诱导子对喜树幼苗中喜树碱含量及色氨酸脱羧酶活性的影响[D]. 西安:西北大学, 2007:31. Mu W. Effect of elicitors on the content of camptothecin and the activity of tryptophan decarboxylase(TDC)in Camptotheca acuminata seedlings[D]. Xi'an:Northwest University, 2007:31.
[13] 阎秀峰, 王洋, 于涛, 等. 喜树叶中喜树碱含量的高效液相色谱分析[J]. 分析测试学报, 2002, 21(2):15-18. Doi:10.3969/j.issn.1004-4957.2002.02.005. Yan X F, Wang Y, Yu T, et al. Determination of camptothecin in leaves of Camptotheca acuminata Decne. by HPLC[J]. Journal of Instrumental Analysis, 2002, 21(2): 15-18.
[14] 潘玉梅, 刘明超, 唐赛春, 等. 光强对三叶鬼针草生长特征的影响[J]. 广西植物, 2012, 32(1): 77-82. Doi:10.3969/j.issn.1000-3142.2012.01.016. Pan Y M, Liu M C, Tang S C, et al. Effect of light intensity on the growth characteristics of Bidenspilosa[J]. Guihaia, 2012, 32(1): 77-82.
[15] 孙中元, 马艳军, 刘杏娥, 等. 弱光环境对高地省藤幼苗生长与光合作用的影响[J]. 西南林业大学学报, 2013, 33(1): 16-21. Doi:10.3969/j.issn.2095-1914.2013.01.003. Sun Z Y, Ma Y J, Liu X E, et al. Effects of low light environment on the growth and photosynthesis of Calamusnam bariensis seedlings[J]. Journal of Southwest Forestry University, 2013, 33(1): 16-21.
[16] 朱延姝, 冯辉. 不同弱光环境下番茄幼苗生长的变化[J]. 辽宁农业科学, 2006(4):16-18. Zhu Y S, Feng H. Changes of tomato seedlings growth under low light density environment[J]. Liaoning Agricultural Sciences, 2006(4): 16-18. Doi:10.3969/j.issn.1002-1728.2006.04.005.
[17] 武维华. 植物生理学[M]. 北京: 科学出版社, 2008:91-92.
[18] Kathju S, Burman U, Garg B.K. Influence of nitrogen fertilization on water relations, photosynthesis, carbohydrate and nitrogen metabolism of diverse pearl millet genotypes under arid conditions[J]. The Journal of Agricultural Science, 2001, 137(3): 307-318.
[19] 张莉, 续九如. 水分胁迫下刺槐不同无性系生理生化反应的研究[J]. 林业科学,2003, 39(4):162-167. Doi:10.11707/j.1001-7488.20030428. Zhang L, Xu J R. Studies on physiological and biochemical responses of Robinia pseudoacacia clones under water stress[J]. Scientia Silvae Sinicae, 2003, 39(4): 162-167.
[20] 霍常富, 孙海龙, 王政权, 等. 光照和氮营养对水曲柳苗木生长及碳-氮代谢的影响[J]. 林业科学, 2009, 45(7):38-44. Doi:10.3321/j.issn:1001-7488.2009.07.007. Huo C F, Sun H L, Wang Z Q, et al. Effects of light and nitrogen on growth, carbon and nitrogen metabolism of Fraxinus maudshurica seedlings[J]. Scientia Silvae Sinicae, 2009, 45(7): 38-44.
[21] 张益锋, 何平, 李桂强, 等. 光强对金荞麦幼苗部分生理指标和生物量的影响[J]. 西南大学学报(自然科学版), 2010, 32(4): 6-11. Zhang Y F, He P, Li G Q, et al. Effect of light intensity on part of the physiological index and biomass of Fagopyrum dibotrys seedlings[J]. Journal of Southwest University(Natural Science Edition), 2010, 32(4): 6-11.
[22] 吴能表, 李琳琳, 杨卫星, 等. 光强对长春花叶片碳氮及次生代谢产物积累的影响[J]. 草业科学, 2014, 31(8): 1508-1514. Doi: 10.11829/j.issn.1001-0629.2013-0584. Wu N B, Li L L, Yang W X, et al. Effects of light intensity on carbon-nitrogen metabolism and secondary metabolite of Catharauthus roseus leaves[J]. Pratacultural Science, 2014, 31(8): 1508-1514.
[23] 皮妍, 蒋科技, 乔守怡, 等. 喜树碱的生物合成途径和代谢调控[J]. 植物生理学通讯, 2009, 45(11): 1051-1060.Doi:10.13592/j.cnki.ppj.2009.11.001. Pi Y, Jiang K J, Qiao S Y, et al. Biosynthesis pathway and metabolic regulation of camptothecin[J]. Plant Physiology Journal, 2009, 45(11): 1051-1060.
[24] 徐步青, 崔永一, 郭岑, 等. 不同光照强度和培养时间下铁皮石斛类原球茎生物量、多糖和生物碱量的动态变化[J]. 中草药, 2012, 43(2): 355-359. Xu B Q, Cui Y Y, Guo C, et al. Dynamic variation of biomass and content of polysaccharide and alkaloidin protocorm like bodies from Dendrobium officinale at different light intensities and incubation time[J]. Chinese Traditional and Herbal Drugs, 2012, 43(2): 355-359.
[25] 郑明, 周冀衡, 黄勇. 光照强度对烤烟烟苗生长和代谢产物含量的影响[J]. 作物研究, 2009,23(3): 181-183. Doi:10.3969/j.issn.1001-5280.2009.03.006. Zheng M, Zhou Y H, Huang Y. Effects of illumination intensity on growth of tobacco seedlings and content of metabolites[J]. Crop Research, 2009, 23(3): 181-183.
[26] 佟璐, 张宝友, 唐中华, 等. 弱光胁迫对大田长春花生物量分配及次生代谢的影响[J]. 植物研究, 2011, 31(2): 227-230. Tong L, Zhang B Y, Tang Z H, et al. Effects of low light stress on the biomass distribution and secondary metabolism of Catharanthus roseus[J]. Bulletin of Botanical Research, 2011, 31(2): 227-230.
[27] 王洋, 戴绍军, 阎秀峰. 光强对喜树幼苗叶片次生代谢产物喜树碱的影响[J]. 生态学报, 2004, 24(6): 1118-1122. Doi:10.3321/j.issn:1000-0933.2004.06.004. Wang Y, Dai S J, Yan X F. Effects of light intensity on secondary metabolite canptothecin production in leaves of Camptotheca acuminata seedlings[J]. Acta Ecologica Sinica, 2004, 24(6): 1118-1122.
[28] 冷平生, 苏淑钗, 王天华, 等. 光强与光质对银杏光合作用及黄酮苷与萜类内酯含量的影响[J]. 植物资源与环境学报, 2002, 11(1): 1-4. Doi:10.3969/j.issn.1674-7895.2002.01.001. Leng P S, Su S C, Wang T H, et al. Effects of light intensity and light quality on photsynthesis, flavonol glycoside and terpene lactone, contents of Ginkgo biloba L. seedlings[J]. Journal of Plant Resources and Environment, 2002, 11(1): 1-4.
[29] 李霞, 于涛, 阎秀峰. 光强对黄檗幼苗生长及抗氧化酶活性的影响[J]. 东北林业大学学报, 2007,35(1):25-30. Doi:10.3969/j.issn.1000-5382.2007.01.008. Li X, Yu T, Yan X F. Effect of light intensity on growth and antioxidant enzyme activities of amurcorktree seedlings[J]. Journal of Northeast Forestry University, 2007, 35(1): 25-30.
[30] 李霞, 王洋, 阎秀峰. 光强对黄檗幼苗三种生物碱含量的影响[J]. 生态学报, 2009, 29(4): 1655-1660. Doi:10.3321/j.issn:1000-0933.2009.04.002. Li X, Wang Y, Yan X F. Effects of light intensity on the contents of three main alkaloids in amurcorktree seedlings[J]. Acta Ecologica Sinica, 2009,29(4):1655-1660.

Last Update: 2016-06-30