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苏云金杆菌与灭幼脲混剂对美国白蛾幼虫 围食膜的破坏研究(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2016年03期
Page:
52-56
Column:
研究论文
publishdate:
2016-05-18

Article Info:/Info

Title:
Destruction of the peritrophic membrane of the larvae of Hyphantria cunea Drury by the mixture of Bt and Chlorbenzuron
Article ID:
1000-2006(2016)03-0052-05
Author(s):
XU Ming12 XU Fuyuan2 WU Xiaoqin1*
1.Co-Innovation Center for Sustanable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China;
2.Jiangsu Forestry Academy, Nanjing 211153, China
Keywords:
mixture of Bt and Chlorbenzuron toxic effect synergism Hyphantria cunea peritrophic membrane(PM) LT50
Classification number :
S763
DOI:
10.3969/j.issn.1000-2006.2016.03.009
Document Code:
A
Abstract:
The ultrathin section was studied by the observation of electron microscope. The results showed that the peritrophic membrane(PM)presented a more obvious multilayer structure, and the surface was evenness and smooth in the 4th instar larvae of Hyphantria cunea Drury. After treated 120h by using the mixture of Bt and Chlorbenzuron on larvae of H. cunea Drury, many white and black spots were formed on PM. Then the spots expanded to patchy pores. Finally thin and brittle, easily broken or fester, inelastic PM fragments were formed. It lead to destruction of the PM and death of larval. The test proved that the damage effect of the treatment of the mixture of Bt and Chlorbenzuron on the PM of the 4 instar larvae of H. cunea was the fastest, and which of the single agent of Bt and Chlorbenzuron on the PM was the second. At the same time, the tests of the value of LT50, LT100 of the 4th instar larvae of H. cunea and the air-spraying control effect by using the mixture of Bt & Chlorbenzuron and its single dose proved that the mortality rate of the mixture was higher than that of the single dose, and the rate of death toxicity of the mixture was faster than single agent. The results showed that the mixture could quickly destroy the PM of the 4th instar larvae of H. cunea, and the toxic effect of the treatment was significantly enhanced.

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Last Update: 2016-06-30