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一种云斑天牛气味结合蛋白的分离纯化(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2016年04期
Page:
164-170
Column:
研究简报
publishdate:
2016-08-30

Article Info:/Info

Title:
Isolation and purification of an odorant binding protein from Batocera lineolata Chevrolat
Article ID:
1000-2006(2016)04-0164-07
Author(s):
ZHUO Zhihang1 YANG Wei1* XU Danping2 YANG Chunping1 YANG Hua1 LIU Chenshan3 XIE Tingting1
1. College of Forestry,Sichuan Agricultural University,Wenjiang 611130, China;
2. College of Food Science, Sichuan Agricultural University,Ya'an 625014, China;
3. College of Agriculture,Chengdu Vocational College of Agricultural Science and Technology,Wenjiang 611130, China
Keywords:
Batocera lineolata Chevrolat odorant binding protein Sephadex G75 protein isolation and purification fluorescent prole
Classification number :
Q969.551.4; S718.7
DOI:
10.3969/j.issn.1000-2006.2016.04.026
Document Code:
A
Abstract:
The fluorescence binding assays and Sephadex G75 chromatography were conducted to investigate the recognition of odorants by Batocera lineolata Chevrolat. Additionally, one of the odorant binding proteins in B. lineolata was isolated and purified. The results showed that the odorant binding protein of B. lineolata could combine with fluorescent probe(N-phenyl-1-naphthylamine, 1-NPN). Besides, twelve kinds of macromolecular crude extract protein(pro. Ⅰ-Ⅻ, Protein Ⅰ-Ⅻ)were found in antenna of B. lineolata. When the 12 kinds of macromolecular crude extract protein were isolated and purified by Sephadex G75, a purified protein(pur-pro. I, purified protein I)was found, and it could also combine with fluorescent probe(N-phenyl-1-naphthylamine, 1-NPN). The relative molecular weight of pur-pro. I was approximately 15.2 ku, and it was pro. Ⅻ(Protein Ⅻ), when contrasted with all kinds of macromolecular crude extract protein. All above, it declared that the isolated and purified protein pur-pro. I was one of the odorant binding proteins in B. lineolata.

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Last Update: 2016-08-30