我们的网站为什么显示成这样?

可能因为您的浏览器不支持样式,您可以更新您的浏览器到最新版本,以获取对此功能的支持,访问下面的网站,获取关于浏览器的信息:

|Table of Contents|

松材线虫毒力及发育转型与细胞自噬关系研究(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2016年04期
Page:
171-176
Column:
研究简报
publishdate:
2016-08-30

Article Info:/Info

Title:
Research on the relationship of autophagy with the virulence and development of Bursaphelenchus xylophilus
Article ID:
1000-2006(2016)04-0171-06
Author(s):
DENG Lina12 WU Xiaoqin1*
1. Co-Innovation Center for the Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Pest Prevention and Management of Invasive Specie, College of Forestry, Nanjing Forestry University, Nanjing 210037, China;
2. School of Ocean and Biological Engineering, Yancheng Institute of Technology, Yancheng 224051,China
Keywords:
Bursaphelenchus xylophilus autophagy virulence development transmission electron microscopy RT-qPCR
Classification number :
S763; Q958.9
DOI:
10.3969/j.issn.1000-2006.2016.04.027
Document Code:
A
Abstract:
In order to identify the autophagy in Bursaphelenchus xylophilus, research on the relationship between autophagy and the different virulence and development of B. xylophilus was carried out in this paper. Transmission electron microscopy(TEM)method was used to identify autophagy in B. xylophilus, and quantitative reverse transcription PCR(qRT-PCR)was adopted to quantify the BxAtg1 and BxAtg8 at different virulence and at different stages of the development of B. xylophilus. The results from TEM observations showed that the process of autophagy existed in B. xylophilus after starvation-induced. The mRNA expression of BxAtg1 was decreased in different virulence nematodes which exposed to long-term starvation for 12, 24 and 36 h. The mRNA expression of BxAtg8 in strongly virulent nematodes was increased in 24 h, but the mRNA expression of BxAtg8 in weakly virulent nematodes was obviously increased in 36 h. And there was increment of both the mRNA expression of BxAtg1 and BxAtg8 in J and Jcompared to the propagtive form of B. xylophilus. Compareing to the morphological characteristics of the different development of Bursaphelenchus xylophilus. These suggest that the mechanism of autophagy activity regulating may be related to the virulence of nematodes and autophagy assisted the nematodes in entering dispersal phase, which improved their ability to adapt to the environment at the same time. This study provided fundamental information the autophagy of B. xylophilus.

References

[1] 叶建仁. 松材线虫病诊断与防治技术[M]. 北京:中国林业出版社, 2010.
[2] 杨宝君. 松材线虫病[M]. 北京: 中国林业出版社, 2003:1-25.
[3] Mamiya Y. Pathology of the pine wilt disease caused by Bursaphelenchus xylophilus[J]. Annu Rev Phytopathol, 1983, 21:201-220. Doi:10.1146/annurev.py.21.090183.001221.
[4] Wingfield M J, Blanchette R A, Nicholls T H, et al. The pine wood nematode: a comparison of the situation in the United States and Japan[J]. Canadian Journal of Forest Research, 1982, 12(1):71-75. Doi:10.1139/x82-010.
[5] 杨振德, 赵博光. 松材线虫行为学研究进展[J]. 南京林业大学学报(自然科学版), 2003, 27(1): 87-92. Doi:10.3969/j.issn.1000-2006.2003.01.020. Yang Z D, Zhao B G. Review on behavior studies of the pine wood nematode[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2003, 27(1): 87-92.
[6] 王玲萍. 松墨天牛生物学特性的研究[J]. 福建林业科技, 2004, 31(3): 23-26. Doi:10.3969/j.issn.1002-7351.2004.03.006. Wang L P. Study on the biological characteristic of Monochamus alternatus Hope[J]. Journal of Fujian Forestry Science And Technology, 2004, 31(3): 23-26.
[7] Reggiori F, Klionsky D J. Autophagy in the eukaryotic cell[J]. Eukaryotic Cell, 2002, 1(1): 11–21.
[8] 张磊. 稻瘟病菌相关致病基因MgATG3、MgATG4和MgATG7的克隆和功能分析[D]. 杭州: 浙江大学, 2007. Zhang L. The molecular characterization and functional analysis of pathogenic related gene MgATG3、MgATG4 and MgATG7 from Magnaporthe grisea[D]. Hangzhou: Zhejiang University, 2007.
[9] Asakura M, Ninomiya S, Sugimoto M, et al. Atg26-mediated pexophagy is required for host invasion by the plant pathogenic fungus colletotrichum orbiculare[J]. The Plant Cell Online, 2009, 21(4):1291-1304. Doi:10.1105/tpc.108.060996.
[10] 李超萍, 林春花, 翟李刚, 等. 橡胶树胶孢炭疽病菌致病相关基因CgATG8的功能分析[J]. 热带作物学报, 2013,34(11):2172-2178. Doi:10.3969/j.issn.1000-2561.2013.11.017. Li C P, Lin C H, Zhai L G, et al. Functional analysis of pathogenic related gene CgATG8 from Colletotrichum gloeosporioides[J]. Chinese Journal of Tropical Crops, 2013, 34((11)):2172-2178.
[11] Veneault-Fourrey C, Barooah M, Egan M, et al. Autophagic fungal cell death is necessary for infection by the rice blast fungus[J]. Science, 2006, 312:580-583. Doi:10.1126/science.1124550.
[12] 朱克恭. 松材线虫病研究综述[J]. 世界林业研究, 1995(4): 28-33. Zhu K G. A review of studies on pine wilt disease caused by Bursaphelenchus xylophilus[J]. World Forestry Research, 1995(4): 28-33.
[13] 张宾,胡春祥,石进,等. 线虫转型发育和寄主识别的化学通讯研究进展[J]. 生态学报, 2013, 33(7): 2003-2012. Doi:10.5846/stxb201112241967. Zhang B,Hu C X,Shi J, et al. Research progress on chemical communication of development and host-finding of nematodes[J]. Acta Ecologica Sinica, 2013, 33(7): 2003-2012.
[14] Alberti A, Michelet X, Djeddi A, et al. The autophagosomal protein LGG-2 acts synergistically with LGG-1 in dauer formation and longevity in C. elegans[J]. Autophagy, 2010, 6(5):622-633. Doi:10.4161/auto.6.5.12252.
[15] Melendez A. Autophagy genes are essential for dauer development and life-span extension in C. elegans[J]. Science, 2003:1387-1391. 10.1126/science.1087782.
[16] 张治宇, 张克云, 林茂松,等. 不同松材线虫群体对黑松的致病性测定[J]. 南京农业大学学报, 2002, 25(2):43-46. Doi:10.3321/j.issn:1000-2030.2002.02.010. Zhang Z Y, Zhang K Y, Lin M S, et al. Pathogenicity determination of Bursaphelenchus xylophilus isolates to Pine thunbergii[J]. Journal of Nanjing Agricultural University, 2002, 25(2):43-46.
[17] 朱丽华, 施学文, 胡玉丹,等. 不同毒力松材线虫杂交后代的繁殖力和致病力的比较[J]. 南京林业大学学报(自然科学版), 2015, 39(1):1-5. Doi:10.3969/j.issn.1000-2006.2015.01.001. Zhu L H, Shi X W, Hu Y D, et al. The fecundity and virulence of offspring crossbred from virulent and avirulent isolates of Bursaphelenchus xylophilus[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2015, 39(1):1-5.
[18] Deng L N, Wu X Q, Ye J R, et al. Identification of autophagy in the pine wood nematode Bursaphelenchus xylophilus and the molecular characterization and functional analysis of two novel autophagy-related genes, BxATG1 and BxATG8[J]. Ijms, 2016, 17(3):279. Doi:10.3390/ijms17030279.
[19] 刘雪莲. 中国松材线虫群体形态学及致病性变异的研究[D]. 南京: 南京林业大学, 2007. Liu X L. Morphology and pathogenic mutation of Bursaphelenchus xylophilus in china[D]. Nanjing: Nanjing Forestry University, 2007.
[20] Levine B, Kroemer G. Autophagy in the pathogenesis of disease[J]. Cell, 2008, 132(1):27-42. Doi:10.1016/j.cell.2007.12.018.
[21] Mizushima N. Autophagy: process and function[J]. Genes Dev, 2007, 21(22):2861-2873. Doi:10.1101/gad.1599207.
[22] 王威, 陈景红, 王新宁, 等. 葡萄籽原花青素诱导人肝癌HepG2细胞凋亡及自噬性死亡[J]. 暨南大学学报(自然科学与医学版), 2011, 32(2): 181-187. Doi:10.3969/j.issn.1000-9965.2011.02.014. Wang W, Chen J H, Wang X N,et al. Grape seed proanthocyanidins induce apoptosis and autophagic cell death in human hepatoma HepG2 cells[J]. Journal of Jinan University(Natural Science & Medicine Edition), 2011, 32(2): 181-187.
[23] 高玉仁, 张德福, 王梁,等. 桧木醇激活膀胱癌J82细胞自噬诱导细胞凋亡[J]. 中国癌症杂志, 2015(5): 365-370. Doi:10.3969/j.issn.1007-3969.2015.05.008. Gao Y R, Zhang D F, Wang L, et al. Hinokitiol induces bladder cancer J82 cells apoptosis via autophagy induction[J]. China Oncology, 2015(5): 365-370.
[24] Shpilka T, Weidberg H, Pietrokovski S, et al. Atg8: an autophagy-related ubiquitin-like protein family[J]. Genome Biol, 2011, 12(7):226. Doi:10.1186/gb-2011-12-7-226.
[25] Xie Z, Nair U, Klionsky D J. Atg8 controls phagophore expansion during autophagosome formation[J]. Mol Biol Cell, 2008, 19(8):3290-3298. Doi:10.1091/mbc.E07-12-1292.
[26] Aladzsity I, Tóth M L, Sigmond T, et al. Autophagy genes unc-51 and bec-1 are required for normal cell size in Caenorhabditis elegans[J]. Genetics, 2007, 177(1):655-660. Doi:10.1534/genetics.107.075762.

Last Update: 2016-08-30