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|Table of Contents|

miR164a及其靶基因PeNAC1相互作用研究(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2016年05期
Page:
29-33
Column:
研究论文
publishdate:
2016-09-30

Article Info:/Info

Title:
Interaction between miR164a and its target PeNAC1
Article ID:
1000-2006(2016)05-0029-05
Author(s):
WANG Haoran LI ShuangshuangLE LinaKUANG HualinHUANG MinrenCHEN Ying*
Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Poplar Germplasm Nursery, Jiangsu Provincial Platform for Conservation and Utilizations for Agricultural Germplasm, Nanjing 210037, China
Keywords:
miR164a Ptc-MIR164a PeNAC1 target gene Populus trichocarpa
Classification number :
S722; Q343
DOI:
10.3969/j.issn.1000-2006.2016.05.005
Document Code:
A
Abstract:
miRNA plays a central role in gene regulations. Many research showed that miR164a targets NAC(NAM/ATAF/CUC)genes involved in multiple biological functions, such as plant development, hormones singal transduction and environmental stimuli. To confirm the interaction between micro164a and its predicted target gene PeNAC1 in poplar, the pre-micro164a sequence was cloned from P. trichocarpa genome DNA by PCR, named Ptc-MIR164a. And according to the results of secondary structure analyses with RNA fold(http://rna.tbi.univie.ac.at/), Ptc-MIR164a can form a typical stem-loop structure that suggests it can be processed into mature miRNA164a in vivo. Then combining the principle of luciferase reporter plasmids used in miRNA target detection in animal cell and the transient expression system of poplar protoplasts, we confirmed the interaction between Ptc-MIR164a and PeNAC1 and established a simple and intuitive method for miRNA target detection in plant cell.

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Last Update: 2016-10-30