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基于SCAR标记的20个樱花品种的分子鉴别(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2016年05期
Page:
34-40
Column:
研究论文
publishdate:
2016-09-30

Article Info:/Info

Title:
SCAR markers-based molecular identification of 20 flowering cherries(Cerasus) cultivars
Article ID:
1000-2006(2016)05-0034-07
Author(s):
ZHAO Qingjie12 LI Haibo1 QU Yan2 WANG Qinghua3 GUO Jia4 XU Liang1 Symbolj@@
1.Zhejiang Forestry Academy, Zhejiang Provincial Key Laboratory of Biological and Chemical Utilization of Forest Resource, Hangzhou 310023, China;
2. School of Landscape, Southwest Forestry University, Kunming 650224, China;
3. East Lake Scenic Area of Wuhan, Wuhan 430074, China;
4. Zhejiang Chengbang Landscape Architecture Company, Hangzhou 310008, China
Keywords:
flowering cherries(Cerasa) SCAR molecualr markers molecualr identification
Classification number :
S685
DOI:
10.3969/j.issn.1000-2006.2016.05.006
Document Code:
A
Abstract:
In the present study, 20 cultivars of flowering cherries(Cerasus), which were widely cultivated in gardens, were identified by developing specific sequence characterized amplified region(SCAR)markers from amplified polymorphic DNA(SAPD)and sequence-related amplified polymorphism(SRAP)techniques. Forty-six polymorphic bands(33 from SAPD and 13 from SRAP)among the 20 studied cultivars were obtained by using 4 SAPD primers and 96 SRAP primers. Fourteen(10 from SAPD and 4 from SRAP)of 46 polymorphic bands were converted into 14 stable and reproducible SCAR markers by designing specific SCAR primers from these sequenced polymorphic bands and by verifying PCR amplification with SCAR primers. The 14 SCAR markers was distributed in 20 different Cerasus cultivars. The cultivars C. serrulata var. lannesiana ‘Albo-rosea', C. serrulata var. lannesiana ‘Superba', C. serrulata var. lannesiana ‘Gioiko', C. serrulata var. lannesiana ‘Kanzan', C. serrulata var. lannesiana ‘Ojochin' and C. serrulata var. lannesiana ‘Asahiyama' possess only one SCAR marker, C.serrulata var. lannesiana ‘Grandifora', Cerasus × sieboldii ‘Beni-yutaka', Cerasus × yedoensis ‘Sakuyahime', C.serrulata var. lannesiana ‘Shujaku' and C. serrulata var. lannesiana ‘Plena-pendula' possess two SCAR markers, C. jamasakura ‘Albo Plena' and C. serrulata var. lannesiana ‘Imose' possess three SCAR markers, C. subhirtella ‘Plena Rosea' and C.subhirtella‘Ujou-shidare' possess four SCAR markers, and C. serrulata var. lannesiana ‘Benigasa', C. serrulata var. lannesiana ‘Kouka', C.serrulata var. lannesiana ‘Hisakura', C. serrulata var. lannesiana ‘Contorta' and C. serrulata var. lannesiana‘Mollis'possess no SCAR markers. These SCAR markers can be used as cultivar-specific DNA fingerprints to promote the identification of 20 Cerasus cultivars, as well as privide molecular data for the establishment of identification system of Cerasus cultivars and for the further study of Cerasus germplasm.

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Last Update: 2016-10-30