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|Table of Contents|

柳杉种子合子胚的愈伤组织诱导及植株再生(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2016年06期
Page:
181-186
Column:
研究简报
publishdate:
2016-11-30

Article Info:/Info

Title:
Callus induction and plant regeneration from zygotic embryos of Cryptomeria fortunei
Article ID:
1000-2006(2016)06-0181-06
Author(s):
DONG Jing SHI Jisen XU Jin*
Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China
Keywords:
Cryptomeria fortunei zygotic embryos callus induction plant regeneration
Classification number :
S791.31; S722.3+7
DOI:
10.3969/j.issn.1000-2006.2016.06.028
Document Code:
A
Abstract:
In this article,immature seeds from 4 families were selected to conduct callus induction and then regeneration plants were obtained by organogenesis in Cryptomeria fortunei by orthogonal design with different types of hormones and concentration. The effect caused by respective factors and interactions on callus induction from zygotic embryos of Cryptomeria fortunei were studied. The following were the results: ① The amount of callus induction from 4 families increased obviously collecting seeds in the mid August. By the late August, the callus induction rate from each zygotic embryos reached the highest, among which family NO. 57 family took the first place(92%). ② There were certain differences between the induction rate by using different hormone combinations. The highest induction rate of callus was that of family NO. 57 family which grew on DCR+6-BA 1.0 mg/L +2,4-D 1.0 mg/L culture medium,and the callus induced was white, dry and of highly dense form, with no needle. ③ Regeneration plants were successfully abtained from callus on the DCR basic medium.

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Last Update: 2016-11-20