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重瓣百合LiSEP3基因克隆与表达分析(PDF/HTML)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2017年01期
Page:
42-48
Column:
研究论文
publishdate:
2017-01-31

Article Info:/Info

Title:
Cloning and expression analysis of gene LiSEP3 in double lily
Article ID:
1000-2006(2017)01-0042-07
Author(s):
Cloning and expression analysis of gene LiSEP3 in double lily
1. College of Biology and Food Engineering, Fuyang Normal College, Fuyang 236037, China;
2. Beijing Key Laboratory of Development and Quality Control of Ornamental Crops, Department of Ornamental Horticulture and Landscape Architecture, China Agricultural University, Beijing 100193, China;
3. College of Agriculture, Liaocheng University, Liaocheng 252059, China
Keywords:
lily MADS-box family gene cloning expression analysis LiSEP3 gene
Classification number :
S682; Q785
DOI:
10.3969/j.issn.1000-2006.2017.01.007
Document Code:
A
Abstract:
【Objetctive】 We cloned LiSEP3 from a double lily and analyzed LiSEP3 expression pattern among different aboveground organs and petals, for probing into SEP3 gene expression pattern in the double flower. 【Method】 LiSEP3 was isolated from double lily cv. ‘Belonica’ by RACE, and protein structure of LiSEP3 was predicted by SMART software. Phylogenetic analysis was performed by MEGA 5.0, subcellular localization of LiSEP3 were detected by particle bombardment. In addition, the expression pattern of LiSEP3 was analyzed among different aboveground organs and petals. 【Result】 LiSEP3 is a member of E class of MADS-box. The open reading frame of LiSEP3 is 729 bp in length, and encodes a protein of 242 amino acids. Protein structure analysis showed that LiSEP3 has a conserved MADS domain, a K-box region and two SEP motifs. Phylogenetic tree analysis suggested LiSEP3 had the highest similarity with monocotyledonous Alstroemeria. Subcellular localization assay revealed LiSEP3 was a nucleus protein. LiSEP3 was abundant in flowers while it couldn’t be detected in stems and leaves. LiSEP3 was expressed in petals from the first whorl to the seventh whorl, and had the highest transcription level in the seventh whorl petals. 【Conclusion】 LiSEP3 belongs to E class of MADS-box family genes and is specifically expressed in flowers and has a higher expression in seventh whorl than that in other whorls. Moreover, the expression pattern of LiSEP3 in flowers is different from its homologous genes in dicotyledons.

References

[1] COEN E S, MEYEROWITZ E M. The war of the whorls—genetic interactions controlling flower development[J]. Nature, 1991, 353(6339):31-37. DOI: 10.1038/353031a0.
[2] THEISSEN G. Development of floral organ identity: stories from the MADS house[J]. Current Opinion in Plant Biology, 2001, 4(1):75-85. DOI: 10.1016/S1369-5266(00)00139-4.
[3] ROUX F, TOUZET P, CUGUEN J, et al. How to be early flowering: an evolutionary perspective[J]. Trends in Plant Science, 2006, 11(8):375-381. DOI:10.1016/j.tplants.2006.06.006.
[4] MESSENGUY F, DUBOIS E. Role of MADS box proteins and their cofactors in combinatorial control of gene expression and cell development[J]. Gene, 2003, 316(2):1-21. DOI: 10.1016/S0378-1119(03)00747-9.
[5] KIM S, KOH J, YOO M J, et al. Expression of floral MADS-box genes in basal angiosperms: implications for the evolution of floral regulators[J]. Plant Journal, 2005, 43:724-744. DOI:10.1111/j.1365-313X.2005.02487.x.
[6] GONG B H, YI J, WU J, et al. LlHSFA1, a novel heat stress transcription factor in lily(Lilium longiflorum), can interact with LlHSFA2 and enhance the thermotolerance of transgenic Arabidopsis thaliana[J]. Plant Cell Reports, 2014, 33(9):1519-1533. DOI: 10.1007/s00299-014-1635-2.
[7] 席梦利, 刘坡, 吴小萍, 等. 百合花发育相关基因LLGLO1的RNAi载体构建[J]. 南京林业大学学报(自然科学版), 2010, 34(1):1-4. DOI: 10.3969/j.issn.1000-2006.2010.01.001. XI M L, LIU P, WU X P, et al. Construction of RNAi vector of LLGLO1 gene related to flower development of Lilium[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2010, 34(1):1-4. DOI: 10.3969/j.issn.1000-2006.2010.01.001.
[8] TONG Z, LI Q H, YANG Y J,et al. Isolation and expression analysis of LoPIP2, a lily (Lilium oriental hybrids)aquaporin gene involved in desiccation-induced anther dehiscence[J]. Scientia Horticulturae, 2013, 164:316-322. DOI: 10.1016/j.scienta.2013.09.022.
[9] SUI J, HE J N, WU J, et al. Characterization and functional analysis of transcription factor LoMYB80 related to anther development in lily(Lilium oriental hybrids)[J]. Journal of Plant Growth Regulation, 2015, 34:1-13. DOI: 10.1007/s00344-015-9489-6.
[10] 吴祝华, 施季森, 池坚, 等. 观赏百合资源与育种研究进展[J]. 南京林业大学学报(自然科学版), 2006, 122: 113-118. DOI:10.3969/j.issn.1000-2006.2006.02.027. WU Z H, SHI J S, CHI J, et al. Research advances on resources and breeding of ornamental lily[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2006, 122: 113-118. DOI:10.3969/j.issn.1000-2006.2006.02.027.
[11] HONMA T, GOTO K. Complexes of MADS-box proteins are sufficient to convert leaves into floral organs[J]. Nature, 2001, 409(6819):525-529. DOI:10.1038/35054083.
[12] PELAZ S, GUSTAFSON-BROWN C, KOHALMI S E, et al. APETALA1 and SEPALLATA3 interact to promote flower development[J]. Plant Journal, 2001, 26(4):385-394. DOI: 10.1046/j.1365-313X.2001.2641042.x.
[13] ZAHN L M, KONG H, LEEBENS-MACK J H, et al. The evolution of the SEPALLATA subfamily of MADS-box genes: a preangiosperm origin with multiple duplications throughout angiosperm history[J]. Genetics, 2005, 169(4):2209-2223. DOI:10.1534/genetics.104.037770.
[14] MELZER R, THEISSEN G. Reconstitution of floral quartets in vitro involving class B and class E floral homeotic proteins[J]. Nucleic Acids Research, 2009, 37(8):2723-2736. DOI: 10.1093/Nar/Gkp129.
[15] IMMINK R G H, TONACO I A N, DE FOLYER S, et al. SEPALLATA3: the ‘glue’ for MADS box transcription factor complex formation[J]. Genome Biology, 2009, 10(2):1-16. DOI: 10.1186/Gb-2009-10-2-R24.
[16] 贾春蕾, 向林, 秦德辉,等. 春兰CgSEP3基因的克隆和表达分析[J]. 西北植物学报, 2014, 34(7):1305-1310. DOI:10.7606/j.issn.1000-4025.2014.07.1305. JIA C L, XIANG L, QIN D H, et al. Expression analysis of CgSEP3 gene from Cymbidium goeringii[J]. Acta Botanica Boreali-Occidentalia Sinica, 2014,34(7): 1305-1310.
[17] 徐启江. 草原龙胆花器官MADS-box基因克隆与表达分析[D]. 哈尔滨:东北林业大学, 2007. XU Q J. Molecular colning and expression analysis of floral organ identity MADS-box genes from lisianthus(Eustoma grandiflorum)[D]. Harbin: Northeast Forestry University, 2007.
[18] 黄赫. 洋葱A、B、C、E功能MADS-box基因的克隆与表达分析[D]. 哈尔滨:东北林业大学, 2013. HUANG H. Molecular colning and expression analysis of A、B、C and E-class MADS-box genes from onion(Allium cepa L.)[D]. Harbin: Northeast Forestry University, 2013.
[19] ANGENENT G C, FRANKEN J, BUSSCHER M, et al. Co-suppression of the petunia homeotic gene fbp2 affects the identity of the generative meristem[J]. Plant Journal, 1994, 5(1):33-44. DOI:10.1046/j.1365-313X.1994.5010033.x.
[20] TZENG T Y, HSIAO C C, CHI P J, et al. Two lily SEPALLATA-like genes cause different effects on floral formation and floral transition in Arabidopsis[J], Plant Physiology, 2003, 133:1091-1101. DOI: 10.1104/pp.103.026997.
[21] 宫本贺. 百合热激转录因子L1HSFA1及其下游热激蛋白L1HSP70响应热胁迫的机制解析[D].北京:中国农业大学, 2014. GONG B H. Mechanism analysis of response to heat stress of LlHAFA1 and its downstream LlHSP70 from lily(Lilium longiflorm)[D]. Beijing:China Agricultural University, 2014.
[22] 徐淑平,卫志明. 基因枪的使用方法介绍[J]. 植物生理学通讯, 1998, 34(1): 41-43. DOI: 10.13592/j.cnki.ppj.1998.01.014. XU S P, WEI Z M. Introduction to method of microprojectile bombardment and its application[J]. Plant Physiology Communications, 1998, 34(1): 41-43.
[23] LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(T)(-Delta Delta C)method[J]. Methods, 2001, 25(4):402-408. DOI: 10.1006/meth.2001.1262.
[24] CUI R, HAN J, ZHAO S, et al. Functional conservation and diversification of class E floral homeotic genes in rice(Oryza sativa)[J]. Plant Journal, 2010, 61(5):767-781. DOI:10.1111/j.1365-313X.2009.04101.x.
[25] TZENG T Y, LIU H C, YANG C H. The C-terminal sequence of LMADS1 is essential for the formation of homodimetgrs for B function proteins[J]. Journal of Biological Chemistry, 2004, 279(11):10747-10755. DOI:10.1074/jbc.M311646200.
[26] CHANG Y Y, CHIU Y F, WU J W, et al. Four orchid(Oncidium Gower Ramsey)AP1/AGL9-like MADS box genes show novel expression patterns and cause different effects on floral transition and formation in Arabidopsis thaliana[J]. Plant Cell Physiology, 2009, 50(8):1425-1438. DOI:10.1093/pcp/pcp087.
[27] THEISSEN G, BECKER A, di ROSA A, et al. A short history of MADS-box genes in plants[J]. Plant Molecular Biology, 2000, 42(1):115-149. DOI: 10.1023/A:1006332105728.

Last Update: 2017-01-30