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松墨天牛的人工饲育技术研究(PDF/HTML)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2017年01期
Page:
199-202
Column:
研究简报
publishdate:
2017-01-31

Article Info:/Info

Title:
Rearing techniques of Monochamus alternatus Hope(Coleoptera: Cerambycidae)on artificial diets
Article ID:
1000-2006(2017)01-0199-04
Author(s):
CHEN Ruixu1 WANG Lujie1 LIN Tao1 WEI Zhiqiang1 WANG Yan2 HAO Dejun1*
1. Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China;
2. Forestry Station of Shanghai, Shanghai 200072, China
Keywords:
Monochamus alternatus artificial diet formula indoor rearing technique
Classification number :
S763; Q969
DOI:
10.3969/j.issn.1000-2006.2017.01.031
Document Code:
A
Abstract:
【Objetcive】 In order to acquire large number of even-aged and consistent physiological status larvae to provide enough insects for involved research, mass rearing techniques of Monochamus alternatus were carried out on artificial diets in the laboratory. 【Method】 Developmental time and activities at different stages were compared among A, B, C three kinds of artificial diets. A(pine sawdust 100 g, agar 40 g, sucrose 20 g, yeast extract 12.5 g, sodium benzoate 2 g, potassium sorbate 1 g, 0.5 mol/L H2SO4 10 mL, H20 800 mL), B(pine sawdust 100 g, agar 40 g, sucrose 20 g, yeast extract 12.5 g, sodium benzoate 2 g, potassium sorbate 1 g, 0.5 mol/L H2SO4 10 mL, cholesterol 1.5 g, ascorbic acid 4 g, casein 20 g, choline chloride 1 g, H20 800 mL), C(pine sawdust 100 g, agar 40 g, sucrose 20 g, yeast extract 12.5 g, sodium benzoate 2 g, potassium sorbate 1 g, 0.5 mol/L H2SO4 10 mL, wheat germ powder 25 g, cholesterol 1.5 g, ascorbic acid 4 g, casein 20 g, choline chloride 1 g, H20 800 mL). 【Result】 When provided with diet A, larval and pupal periods were(90.6±9.3)d and(11.9±1.3)d respectively, pupation rate was 6.4% and emergence rate was 66.7%. Pupal weights were(0.37±0.03)g for females and(0.30±0.04)g for males, and adult weights were(0.27±0.03)g for females and(0.21±0.02)g for males after 7 days emergence. When provided with diet B, larval and pupal periods were(71.9±4.0)d and(12.8±0.7)d respectively, pupation rate was 18% and emergence rate was 75.8%. Pupal weights were(0.33±0.02)g for females and(0.27±0.03)g for males, and adult weights were(0.29±0.02)g for females and(0.22±0.03)g for males after 7 days emergence. When provided with diet C, larval and pupal periods were(71.25±2.01)d and(11.12±0.34)d respectively, pupation rate was 58.33% and emergence rate was 88.10%. Pupal weights were(0.63±0.03)g for females and(0.49±0.03)g for males, and adult weights were(0.53±0.03)g for females and(0.43±0.03)g for males after 7 days emergence. The results showed that diet C was confirmed to be the optimal diet according to comparison of developmental time and activities among different modified diets. 【Conclusion】 It is suggested that diet C is superior to diet A and B, and can be a standard diet to laboratory population for further research work.

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Last Update: 2017-01-30