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硫化氢对白桦悬浮细胞中多胺和次生代谢物积累的影响(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2017年06期
Page:
26-32
Column:
研究论文
publishdate:
2017-11-30

Article Info:/Info

Title:
The effect of H2S on the accumulation of polyamines and secondary metabolites in birch suspension cells
Article ID:
1000-2006(2017)06-0026-07
Author(s):
WANG Ziqing LI Feijie GUO Yuting YU Zhaohui MA Yubin ZHOU Wenyang FAN Guizhi*
College of Life Sciences, Northeast Forestry University, Harbin 150040, China
Keywords:
Keywords:birch(Betula platypylla Suk.) suspension cells hydrogen sulfide polyamines secondary metabolites
Classification number :
S792.153
DOI:
10.3969/j.issn.1000-2006.201610011
Document Code:
A
Abstract:
【Objective】The purpose of this study was to clarify the effect of hydrogen sulfide(H2S)on the accumulation of polyamines and secondary metabolites in birch suspension cultures. 【Methods】Different concentrations of H2S donor sodium hydrosulfide(NaHS)were added to a birch suspension culture. High performance liquid chromatography and colorimetric assays were used to analyze the levels of different forms of polyamines, enzyme activity of arginine decarboxylase(ADC)and polyamine oxidase(PAO), and the levels of flavonoids, polyphenols and triterpenoids. 【Result】 The total polyamine content of birch suspension cultures increased initially and then decreased after NaHS treatment. Among the cells, the highest level of putrescine and spermidine was at 1 mmol/L NaHS after 24 h treatment, with increasing rates of 30.51% and 12.37% compared to the control, respectively. With the exception of perchloric acid(PCA)-insoluble spermidine conjugate, the content of free-, PCA-soluble and PCA-insoluble putrescines and conjugates increased after NaHS treatment. Similarly, NaHS treatment enhanced the activity of ADC and PAO, with increasing ranges of 15.91%-26.33% and 137.5%-250.12%, respectively. Treatment on birch suspension cell with 1 mmol/L NaHS and different concentrations of putrescine, spermidine, and spermine increased the polyphenol, flavonoid and triterpenoid levels, respectively. Polyphenol, flavonoid and triterpenoid reached maximum levels after combined treatment with 5 mmol/L putrescine and NaHS; the levels increased by 26.69%, 13.22% and 4.55%, compared to NaHS treatment, respectively. 【Conclusion】 These results indicate that H2S treatment increased the levels of polyamines and secondary metabolites, and the combined treatment with H2S and polyamines further enhanced the accumulation of secondary metabolites in birch suspension cultures.

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