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|Table of Contents|

刺梨黄酮的半仿生法提取工艺及组分研究(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2017年06期
Page:
47-53
Column:
研究论文
publishdate:
2017-11-30

Article Info:/Info

Title:
Semi-bionic extraction of flavonoids from Rosa roxburghii Tratt.
Article ID:
1000-2006(2017)06-0047-07
Author(s):
ZHANG Huihui12 LIU Longyun12 WU Caie13* LI Tingting13 FAN Gongjian13 YING Ruifeng13
1. Co-Innovation Centre for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China; 2.College of Forestry, Nanjing Forestry University, Nanjing 210037,China; 3.College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, China
Keywords:
Keywords:Rosa roxburghii Tratt. flavonoid semi-bionic extraction response surface methodology
Classification number :
TS201.4
DOI:
10.3969/j.issn.1000-2006.201512021
Document Code:
A
Abstract:
【Objective】The optimal conditions for the semi-bionic extraction(SBE)of flavonoids from Rosa roxburghii Tratt. were identified. 【Method】Response surface methodology(RSM)was carried out to investigate the effects of ethanol concentration, extraction temperature, solid-liquid ratio, and extraction time on the yield of flavonoids from Rosa roxburghii Tratt. 【Result】The results showed that the optimal conditions for SBE were 80% ethanol, solid-to-liquid ratio of 1:13.2(mass volume ratio), and extraction at 90 ℃ for 80-40-40 min; the extraction yield of flavonoids under these conditions was 27.70 mg/g. The antioxidant activity was significantly higher than that in the case of Tween auxiliary extraction and ultrasonic-assisted extraction. Component I and Component II were separated by semi-preparing liquid chromatography, and their retention times agreed with those of quercetin and kaempferol. 【Conclusion】SBE is superior to the traditional ethanol extraction, and in combination with RSM, offers better yields of purified quercetin and kaempferol.

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