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落叶型冬青雌、雄株茎段的组织培养技术(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2017年06期
Page:
181-186
Column:
研究简报
publishdate:
2017-11-30

Article Info:/Info

Title:
Micropropagation of Ilex verticillata axillary buds from male and female seedlings
Article ID:
1000-2006(2017)06-0181-06
Author(s):
CHEN Xi12 FAN Jialu13 WANG Baoteng13 WANG Ruiqi12 YUAN Binling12 WEI Zihan12 CHEN Ying12*
1. Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China; 2. College of Biology and Environment, Nanjing Forestry University; 3. College of Forestry, Nanjing Foresty University, Nanjing 210037, China
Keywords:
Keywords:Ilex verticillata micropropagation male and female seedlings axillary buds
Classification number :
S722.8
DOI:
10.3969/j.issn.1000-2006.201704002
Document Code:
A
Abstract:
【Objective】Establish a micropropagation system for male and female of Ilex verticillata L. plant(Winterberry).【Method】In this work, we optimized the culture mediums of axillary bud induction, clustered bud proliferation and shoot rooting by using semi-lignied buds of male and female seedling and adding different regulators such as 6-benzylaminopurine(6-BA), indole-3-butyric acid(IBA), naphthaleneacetic acid(NAA), and N-(2-chloro-4-pyridyl)-N'-phenylurea(CPPU)in vitro.【Result】The optimal method for sterilization of explants was treatment with 70% alcohol for 30 s, and 0.1% HgCl2 for 8 min; the optimal media for axillary bud induction of female and male explants were MS+0.5 mg/L 6-BA+0.2 mg/L IBA and MS + 0.5 mg/L 6-BA + 0.5 mg/L IBA, and this treatment resulted in axillary bud germination rates up to 93.3% and 83.3%, respectively. Furthermore, the optimal media for female and male multiple shoot clumps were MS + 1.0 mg/L CPPU + 0.2 mg/L NAA and MS + 1.0 mg/L CPPU + 0.1 mg/L NAA, this treatment resulted in proliferation rates up to 3.48 and 1.25, respectively. The shoots were induced to form roots on a 1/2MS + 0.4 mg/L NAA medium, and the rooting rates of females and males were only 26.7% and 20.0%, which increased to 66.7% and 46.7%, respectively, when a rooting agent was added to the medium. 【Conclusion】The establishment of this tissue culture system, especially the male plant tissue culture, provides technical support for the rapid propagation of Ilex verticillata species.

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