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PEG和GA3引发处理对老化毛竹种子理化特性的影响(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2018年02期
Page:
40-46
Column:
研究论文
publishdate:
2018-03-20

Article Info:/Info

Title:
Effects of PEG and GA3 priming on the physiological and biochemical characteristics of aged moso bamboo(Phyllostachys edulis)seeds
Article ID:
1000-2006(2018)02-0040-07
Author(s):
CAI Chunju1 FAN Shaohui1* CAO Banghua2 ZHAO Jiancheng13 LIU Feng12
1. International Centre for Bamboo and Rattan Key Laboratory of Science and Technology of Bamboo and Rattan, State Forestry Administration, Beijing 100102, China; 2. College of Forestry, Shandong Agricultural University, Tai'an 271018, Chian; 3. Zhejiang Academy Forestry, Hangzhou 310023, China
Keywords:
Keywords:Phyllostachys edulis seed vigor polyethylene glycol(PEG) gibbereilin(GA3) seed aging
Classification number :
S722
DOI:
10.3969/j.issn.1000-2006.201701021
Document Code:
A
Abstract:
【Objective】The viability of moso bamboo(Phyllostachys edulis)seeds often decreases quickly during the natural storage process, resulting in a short life of seeds, so it is important to enhance the vigor and germination rate of moso bamboo seeds for storage, production, and utilization. The objective of the present study was to investigate the effects of polyethylene glycol(PEG)and gibbereilin(GA3)priming on the physiological and biochemical characteristics of aged moso bamboo seeds, and explore optional methods for improving the vigor of aged seeds.【Method】High temperature(40 ℃)and high humidity(95%)were used to simulate the natural aging process of the seeds, and different vigor levels of moso bamboo seeds were selected. Then, the selected seeds were primed by polyethylene glycol(PEG)and gibberellic acid(GA3), and the physiological and biochemical characteristics of aged moso bamboo seeds were tested. 【Result】With an increased degree of aging, the relative conductivity and soluble sugar, MDA and abscisic acid(ABA)contents increased, and protective enzyme(SOD, POD, and CAT)activities and soluble protein, GA3, indole acetic acid(IAA)and GA3/ABA contents decreased. Furthermore, the higher the degree of seed aging, the more obvious the improvement of seed vigor was. Compared to the control, PEG and GA3 treatments could significantly decrease the relative conductivity and MDA content to reduce the level of membrane lipid peroxidation and protect the integrity of the membrane. The two treatments could also increase the protective enzyme activities and soluble protein, GA3, IAA and GA3/ABA contents, as well as decrease the ABA content. 【Conclusion】The proper PEG(soaking for 24 hours with a concentration of 20% at 20 ℃)and GA3(soaking for 12 hours with a concentration of 500 mg/L at 25 ℃)priming treatments could significantly enhance the seed vigor of aged moso bamboo seeds, and promote seed germination. These results could provide reference and technical guidance for moso bamboo seedling breeding.

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Last Update: 2018-06-12