我们的网站为什么显示成这样?

可能因为您的浏览器不支持样式,您可以更新您的浏览器到最新版本,以获取对此功能的支持,访问下面的网站,获取关于浏览器的信息:

|Table of Contents|

山樱花群体遗传多样性的SSR分析(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2018年05期
Page:
25-31
Column:
研究论文
publishdate:
2018-09-15

Article Info:/Info

Title:
Genetic divetsity of Cerasus serrulata populations assessed by SSR markers
Article ID:
1000-2006(2018)05-0025-07
Author(s):
YI Xiangui CHEN Jie YOU Luxiang CONG Rui WANG HuachenDUAN Yifan WANG Xianrong*
Co-Innovation Center for the Sustainable Forestry in Southern China,College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China
Keywords:
Cerasus serrulata SSR genetic diversity genetic structure
Classification number :
S718.46
DOI:
10.3969/j.issn.1000-2006.201702036
Document Code:
A
Abstract:
【Objective】Cerasus serrulata(Lindley)Loudon, belonging to the Cerasus genus in the Rosaceae family,possesses renowned ornamental value along with rich and superior genetic characters for developing new flowering cherry cultivars. The present study aimed to reveal its genetic diversity and provide a theoretical basis for protecting and utilizing good C. serrulata resources.【Method】SSR markers were used to evaluate the genetic diversity of 224 C. serrulata indivi-duals from eight populations.【Result】A total of 113 alleles were detected from 17 pairs of SSR primes. The percentage of polymorphic loci was 92.65%. Nei's genetic diversity indexes at the species and population level were, respectively, 0.634 and 0.498. The Shannon diversity index was 0.939. Furthermore, the structure analysis showed that the eight po-pulations were from three different gene pools and the results predominantly agreed with the principal coordinate analysis.【Conclusion】SSR markers were used to effectively conduct a genetic diversity analysis of C. serrulata and revealed the high genetic diversity within and between C. serrulata populations.

References

[1] 俞德浚,李朝銮.中国植物志:38卷[M].北京:科学出版社, 1986: 41-87.
[2] LI C L, BRUCE B. Flora of China(9)[M]. Beijing: Science Press, Missouri: Botanical Garden Press, 2003: 404-420.
[3] 王贤荣.中国樱花品种图志[M].北京:科学出版社, 2014.
WANG X R. An illustrated monograph of cherry cultivars in China[M]. Beijing: Science Press, 2014.
[4] 李蒙. 山樱花高海拔居群生态学特征及组织培养[D].南京:南京林业大学, 2013.
LI M. Ecological characteristics and tissue culture of the high altitude population of Cerasus serrulata[D]. Nanjing: Nanjing Forestry University, 2013.
[5] 李蒙,伊贤贵,王华辰,等.山樱花地理分布与水热环境因子的关系[J].南京林业大学学报(自然科学版),2014,38(S1):74-80. DOI:10.3969/j.issn.1000-2006.2014.S1.016.
LI M,YI X G,WANG H C, et al.Studies on the relationship between Cerasus serrulata distribution region and the environmental factors[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2014, 38(S1): 74-80.
[6] 李祯,伊贤贵,顾宇,等. 山樱花基因组大小的测定[J]. 南京林业大学学报(自然科学版),2014,38(S1):17-19. DOI:10.3969/j.issn.1000-2006.2014.S1.004.
LI Z, YI X G, GU Y, et al. The genome size determination of Cerasus serrulata[J]. Journal of Nanjing Forestry University(Natural Sciences Edition),2014,38(S1):17-19.
[7] KOEHNE E. Plantae Wilsonianae: Part 1[M]. Cambridge: The University Press, 1912: 59, 226-273.
[8] WILSON E H. The cherries of Japan: No.7[M]. Massachusetts: Arnold arboretum publication, 1916.
[9] 陈洁.山樱花居群遗传多样性的SSR分析[D].南京:南京林业大学,2016.
CHEN J. Genetic diversity analysis of Cerasus serrulata populations based on SSR markers[D]. Nanjing: Nanjing Forestry University, 2016.
[10] 吕月良.福建山樱花群体遗传多样性、繁育技术体系和育种策略研究[D].南京:南京林业大学, 2006.
LÜ Y L. Genetic diversity and breeding strategy of Cerasus campanulata Maxim.[D].Nanjing:Nanjing Forestry University, 2006.
[11] 苏倩.福建山樱花种源遗传多样性研究[D].南京:南京林业大学, 2007.
SU Q.Genetic diversity among populations of Cerasus campanulata[D].Nanjing: Nanjing Forestry University, 2007.
[12] TSUDA, KIMURA M, KATA S, et al. Genetic structure of Cerasus jamasakura, a Japanese flowering cherry, revealed by nuclear SSRs: implications for conservation[J]. Plant Res, 2009, 122: 367-375. DOI:10.1007/s10265-009-0224-x.
[13] 商韬,王贤荣,南程慧,等. 基于SSR标记的迎春樱自然居群遗传多样性分析[J]. 甘肃农业大学学报, 2013, 48(6):104-109. DOI:10.13432/j.cnki.jgsau.2013.06.021.
SHANG T,WANG X R, NAN C H, et al. Genetic diversity in natural populations of Cerasus discoidea based on SSR markers[J]. Journal of Gansu Agricultural University,2013,48(6):104-109.
[14] 张琼.樱属观赏品种资源调查及部分种与品种SSR分析[D].南京:南京林业大学, 2013.
ZHANG Q. Study on the cultivar resources investigation of flowering cherry(Cerasus)in China and analysis of particle specises and cultivars Using SSR markers[D]. Nanjing: Nanjing Forestry University, 2013.
[15] YOSHIAKI T, SANEYOSHI U, SHURI K, et al. Development of 13 EST-SSRs for Cerasus jamasakura and their transferability for Japanese flowering cherries[J]. Conservation Genetics, 2009, 10: 685-688.
[16] YAMAMOTO T, MOCHIDA K, IMAI T, et al. Microsatellite markers in peach [Prunus persica(L.)Batsch] derived from an enriched genomic and cDNA libraries[J]. Molecular Ecology Notes, 2002, 2: 298-301. DOI:10.1046/j.1471-8286.2002.00242.x.
[17] OHTA S, KATSUKI T, TANAKA T, et al. Genetic variation in flowering cherries(Prunus subgenus Cerasus)charaterized by SSR markers[J]. Breeding Science, 2005, 55: 415-424.
[18] CIPRIANI G, LOT G, HUANG W G, et al. AC/GT and AG/CT microsatellite repeats in peach [Prunus persica(L.)Batch]: isolation, characterization and cross-species amplification in Prunus[J]. Theoretical and Applied Genetics, 1999, 99: 65-72.
[19] TESTOLIN R, MARRAZZO T, CIPRIANI G, et al. Microsatellite DNA in peach(Prunus persica L. Batsch)and its use in fingerprinting and testing the genetic origin of cultivars[J]. Genome, 2000, 43: 512-520. DOI:10.1139/g00-010.
[20] MNEJJA M, GARCIA-MAS J, HOWAD W, et al. Simple-sequence repeat(SSR)markers of Japanese plum(Prunus salicina Lindl.)are highly polymorphic and transferable to peach and almond[J]. Molecular Ecology Notes, 2004, 4(2): 163-166. DOI:10.1111/j.1471-8286.2004.00603.x.
[21] DIRLEWANGER E, COSSON P, TAVAUD M, et al. Development of microsatellite markers in peach [Prunus persica(L.)Batsch] and their use in genetic diversity analysis in peach and sweet cherry(Prunus avium L.)[J]. Theoretical and Applied Genetics, 2002, 105(1): 127-138. DOI:10.1007/s00122-002-0867-7.
[22] SCHUELER S,TUSCH A, SCHUELER M, et al. Characterization of microsatellites in wild and sweet cherry(Prunus avium L.)-markers for individual identification and reproductive processes[J]. Genome, 2003, 46(1): 95-102. DOI:10.1139/g02-107.
[23] LINDLEY J. Transactions of the horticultural Society of London[M]. London: Horticultural Society, 1828,7:238.
[24] IWATSUKI K, BOUFFORD D E, OHBA H. Flora of Japan: Vol. IIb[M]. Tokyo: Kodansha, 2001.
[25] KATO S, IWATA H, TSUMURA Y, et al. Genetic structure of island populations of Prunus lannesiana var. speciosa revealed by chloroplast DNA, AFLP and nuclear SSR loci analyses[J]. Journal of Plant Research, 2011, 124(1):11-23. DOI:10.1007/s10265-010-0352-3.
[26] 南程慧.迎春樱居群变异与繁殖生物学研究[D].南京:南京林业大学, 2012.
NAN C H. Study on population variation and reproductive biology of Cerasus discoidea Yü et Li[D]. Nanjing: Nanjing Forestry University, 2012.
[27] 何恒流, 蔡宇良, 高天翔, 等. 陕西7个毛樱桃自然居群遗传多样性的SSR评价[J]. 西北农林科技大学学报(自然科学版), 2015,43(6):193-198. DOI:10.13207/j.cnki.jnwafu.2015.06.021.
HE H L,CAI Y L,GAO T X, et al. SSR evaluation of genetic diversity of seven wild Prunus tomentosa populations in Shaanxi[J]. Journal of Northwest A & F University(Nature Science Edition),2015,43(6):193-198.
[28] 曹东伟. 李属樱亚属植物分子亲缘地理学研究[D].西安:西北大学, 2006.
CAO D W. Molecular Phylogeography of Prunus subgenus Cerasus[D]. Xi'an: Northwest University, 2006.
[29] SCHAAL B A, HAYWORTH D A, OLSEN K M. Phylogeographic studies in plants: problems and prospects[J]. Molecular Ecology, 1998, 7:465-475. DOI:10.1046/j.1365-294x.1998.00318.x.
[30] NAGEL J C, CECONI D E, POLETTO I, et al. Historical gene flow within and among populations of Luehea divaricata in the Brazilian Pampa.[J]. Genetica, 2015, 143(3):317-329. DOI:10.1007/s10709-015-9830-9.

Last Update: 2018-09-15