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金花茶杂交种子无菌苗的组培快繁研究(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2018年05期
Page:
32-38
Column:
研究论文
publishdate:
2018-09-15

Article Info:/Info

Title:
Tissue culture and rapid propagation of sterile seedling of Camellia nitidissima C. W. Chi hybrid germplasm
Article ID:
1000-2006(2018)05-0032-07
Author(s):
WU Lijun1 GAO Nan1 CHEN Da1 CHEN Wenrong2
1. Fujian Academy of Forestry Sciences, Fuzhou 350012, China; 2. Laizhou Country Forest Farm of Fujian Province, Nanping 353004, China
Keywords:
Camellia nitidissima C. W. Chi hybrid germplasm sterile seedling tissue culture and rapid propagation proliferation rooting rate transplantation survival rate
Classification number :
S723.1+32.1; S723.2
DOI:
10.3969/j.issn.1000-2006.201709042
Document Code:
A
Abstract:
【Objective】To establish a technological system of tissue culture and rapid propagation of hybrid germplasm of Camellia nitidissima C.W. Chi that would provide technical support for the preservation and rapid propagation of elite hybrid germplasm of C. nitidissima C.W. Chi.【Method】With sterile seedlings from hybrid seeds of C. impressinervis Chang et S.Y. Liang♀× C. japonica ‘Dormi'♂(S1), C. chrysanthoides ‘Huangzun'♀ × C. chrysantha var. longistyla ♂(S2), and C. chrysantha var. longistyla ♀ × C. amplexicaulis ♂(S3)as material, we determined the difference in induction rate from different explants, including the apical bud, stem section, and radicle of a sterile seedling, effects of 3 hybrids, effects of 4 basic media including MS, B5, Hyponex No. 1, and 1/2MS+Hyponex No. 1, effects of concentration combinations of different plant growth regulators(PGRs), including 6-BA, IBA, and NAA on the proliferation and rooting of shoots, effects of sucrose concentration on rooting rate of shoots, and the difference in transplantation survival rate of 3 hybrids on 4 substrates, including pure yellow soil, 3Vpure yellow soil+1Vvermiculite, 3Vpure yellow soil+1Vperlite , and 3Vpure yellow soil+1Vcoconut .【Result】The average induction rate of apical bud and stem section of the 3 hybridized combinations was 12% and 100%, respectively. The difference in proliferation times of shoots was significant for the 3 hybridized combinations, the 4 basic media, and the different concentrations of 6-BA. The proliferation times of S1,S2 and S3 were higher at 7.4, 6.6 and 7.5, respectively, on the subculture medium B5+6-BA 2.5 mg/L+IBA 0.05 mg/L+30 g/L sucrose for 40 d. The shoots grew poorly with falling leaves on the basic medium MS for several generations of multiplication. The difference in the rooting rate of the 3 hybrids was significant on concentration combinations of different PGRs and sucrose concentration, and the higher the sucrose concentration, above 10 g/L, the lower the rooting rate. A higher rooting rate for S1,S2 and S3 was obtained on the medium 1/2 B5+ABT 1.0 mg/L+sucrose 5 g/L, at 92%, 85% and 89%, respectively. The difference in the transplantation survival rate of shoots was significant on the 4 substrates, with the average survival rate of S1, S2 and S3 reaching 94.0% on substrote of 3Vyellow soil+1Vvermiculite after hardening for 30 d.【Conclusion】Stem sections of sterile seedlings were the best explants for tissue cultures of the 3 hybridized combinations of C. nitidissima C.W. Chi. The best subculture and rooting medium was B5+6-BA 2.5 mg/L+IBA 0.05 mg/L+30 g/L sucrose and 1/2 B5+ABT 1.0 mg/L+sucrose 5 g/L, respectively. The optimum transplant mixture was 3Vyellow soil+1Vvermiculite. Tissue culture technology was the best preservation method and method for plantlet regeneration for the elite hybrid germplasm of C. nitidissima C.W. Chi.

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Last Update: 2018-09-15