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拟松材线虫组织蛋白酶基因的RNA干扰及功能研究(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2018年05期
Page:
65-70
Column:
研究论文
publishdate:
2018-09-15

Article Info:/Info

Title:
Study on function of cathepsin bmcath1 in Bursaphelenchus mucronatus using method of RNA interference
Article ID:
1000-2006(2018)05-0065-06
Author(s):
PAN Yanyi12 HUANG Lin1 WU Xiaoqin1*
1. Co-Innovation Center for the Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, College of Forestry, Nanjing Forestry University, Nanjing 210037, China; 2. Huai'an Customs, Huai'an 223001,
Keywords:
Bursaphelenchus mucronatus cathepsin gene RNA interference reproduction ability pathogenicity
Classification number :
S763.1
DOI:
10.3969/j.issn.1000-2006.201612052
Document Code:
A
Abstract:
【Objective】This study aimed to analyze the function of cathepsin bmcath1 in Bursaphelenchus mucronatus.【Method】 Nematodes of B. mucronatus were treated with double-strand RNA(dsRNA)of bmcath1. Reproduction ability, feeding speed, sex ratio, body size, and pathogenicity were analyzed.【Result】Compared with the untreated B. mucronatus, the reproduction ability of nematodes treated by dsRNA of bmcath1 was increased by 75%. The dsRNA-treated nematodes also displayed a quicker feeding speed and stronger pathogenicity. The sex ratio of dsRNA-treated nematodes was changed from ♂3:♀5 to ♂6:♀13, and the percentage of adult nematodes increased from 8% to 19%.【Conclusion】Cathepsin bmcath1 has a significant effect on reproduction ability and pathogenicity in B. mucronatus.

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Last Update: 2018-09-15