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基于流式细胞技术的两种槭属植物基因组大小测定(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2018年05期
Page:
201-205
Column:
研究简报
publishdate:
2018-09-15

Article Info:/Info

Title:
Estimation of genome size of two Acer Linn. by flow cytometry
Article ID:
1000-2006(2018)05-0201-05
Author(s):
MA Qiuyue1 LI Shushun1 MA Xiang2 WEN Jing1 ZHU Lu1 TANG Ling1 LI Rui1 GUO Wei3 LI Qianzhong1*
1.Institute of Leisure Agriculture in Jiangsu Academy of Agricultural Sciences, Nanjing 210014,China; 2. Jintan Bureau of Agricultural and Forestry, Changzhou 213200, China; 3. Institute of Germplasm Resources and Biotechnology, Nanjing 210014, China
Keywords:
Acer Linn. A. palmatum A. truncatum genome size flow cytometry
Classification number :
S687.9; S722
DOI:
10.3969/j.issn.1000-2006.201711030
Document Code:
A
Abstract:
【Objective】Acer plants have potential value as ornamental plants and as a source of medicinal compounds. However, their use is hindered by a lack of molecular biology and genomic data. Presently, flow cytometry was used to examine Acer palmatum Thunb. and A. truncatum Bunge genome sizes, to provide foundational data for cytogenetics and genomics studies of Acer Linn.【Method】Fresh leaves of A. palmatum Thunb and A. truncatum were collected. LB01 and PI were used as isolation buffer. Zea mays L. CE-777 was used as the internal standard for the flow cytometry analyses. 【Result】Samples and the internal standard were better dissociated by the LB01 solution, with particles clearly distinguished and concentrated without overlapping peaks. The genome size of A. palmatum and A. truncatum was 0.840 4 and 0.756 0 pg, respectively.【Conclusion】Optimized sample processing and flow cytometry permitted the determinations of the genome sizes of A. palmatum Thunb and A. truncatum Bunge. The optimized processing and results enrich the Acer gene database and provide foundational data for investigations of origin and evolution of species, and genome sequencing.

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Last Update: 2018-09-15