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|Table of Contents|

茉莉酸甲酯对干旱及复水下落叶冬青苗叶片抗氧化水平的影响(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2018年06期
Page:
35-43
Column:
研究论文
publishdate:
2018-12-15

Article Info:/Info

Title:
The effects of methyl jasmonic acid on leaf antioxidative capacity of Ilex verticillata L. seedlings under drought and re-watering
Author(s):
CHEN Yanqiong12 SHEN Yang12 FAN Jialu13 CHEN Xi12 XU Xinyue13 ZHANG Di13 QIAN Gang13 CHEN Ying12*
(1. Co-Innovation Center for the Sustainable Foresity in Southern China, Nanjing Forestry University, Nanjing 210037, China; 2. College of Biology and Environment, Nanjing Forestry University, Nanjing 210037, China; 3. College of Forestry, Nanjing Forestry University, Nanjing 210037, China)
Keywords:
Ilex verticillata L. drought methyl jasmonate antioxidative capacity alleviating effect
Classification number :
S725; Q945.78
DOI:
10.3969/j.issn.1000-2006.201712035
Document Code:
A
Abstract:
【Objective】Ilex verticillata L.(winterberry, North America holly)is a deciduous shrub and dioecism, and also an important ornamental and commercial species owing to its red fruits in winter. The study of drought treatment to Ilex verticillata seedlings will provide reference for expanding this species scope of cultivation area.【Method】The effects of methyl jasmonate(MJ)on antioxidative capacity in the leaves by spraying MJ, drought and re-watering to seedlings including control, 500 μmol/L MJ, drought, 500 μmol/L MJ +drought(drought+MJ)treatments were investigated. 【Result】Drought significantly reduced leaf water content,pigment(chlorophyll a, b and carotenoids)content while foliar spray with methyl jasmonate under drought increased all above index levels compared to the control. Drought signifi-cantly increased the contents of H2O2, superoxide radical(O·-2)and malondialdehyde(MDA), and attained to high peak on the 12th day treatment, respectively, while application MJ significantly reduced the levels of these parameters under drought stress. Drought affected antioxidative enzyme activities and antioxidant contents differently: superoxide dismutase(SOD)activity was observed decreasing firstly then increasing, however peroxidase(POD), ascorbate peroxidase(APX), glutathione reductase(GR)activities and AsA or GSH content were increased with the drought time extension, and achieved to high peak at 8th day(GR and GSH)or 12th day(POD, APX and AsA), respectively. On the other hand, application MJ under drought stress, POD, APX activities and AsA content at the 8th day, GR activity and GSH content at the 3th day reached high peaks,respectively. Afterward, these antioxidative enzymes and antioxidants correspondingly restored to the low levels with H2O2, and O·-2 level decreasing. Ilex verticillata seedlings could recover under re-watering for 6 days after Drought+MJ treatment for 12 days, but the opposite result was found under single drought treatment. 【Conclusion】 The application MJ could early and quickly activate the antioxidant protection systems, reduce oxidative stress, relieve the injury of the drought stress and improving drought tolerance of Ilex verticillata seedlings.

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Last Update: 2018-11-30