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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:

2018年06期

Page:

128-136

Column:

研究论文

publishdate:

2018-12-15

Article Info:/Info

Title:

Isolation, screening and identification of endophytic bacteria from Cinnamomum camphora that promote growth and antagonistic pathogen

Author(s):

LU Lanxiang¹;  JIANG Mingming¹; WANG Yan²; ZHANG Yuefeng²; ZHANG Hongliang²; YE Jianren^1*

(1. College of Forestry, Nanjing Forestry University, Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing 210037,China; 2. Shanghai Forestry Station, Shanghai 200072, China)

Keywords:

camphor tree(Cinnamomum camphora);  endogenous bacteria;  antagonism;  phosphate-solubilizing;  IAA produce;  salt resistance;  Microbacterium resistens;  Bacillus megaterium

Classification number :

S76

DOI:

10.3969/j.issn.1000-2006.201802022

Document Code:

A

Abstract:

【Objective】Determine the underlying cause of chlorosis in urban greening and canker of Cinnamomum camphora (L.)Presl. 【Method】 We collected the branches and leaves of camphor-tree of Shanghai area, and aimed to isolate endophytic bacterium from camphor-tree. The isolated strains were screened and identified. They could antagonize pathogenic fungi of camphor-tree via phosphorus-solubilizing ability. The endogenous bacteria were isolated from healthy branches and leaves of camphor-tree by tissue slicing method or tissue homogenation method. The endophytic bacteria were screened against the pathogenic fungi in plate for determining the effect of resolved inorganic phosphorus and organic phosphorus by Molybdenum antimony colorimetric method, the effect of indole acetic acid(IAA)production by Salkowski colorimetric method, and its salt resistance.【Result】 Fifty candidate strains were isolated from camphor-tree, of which 11 strains were antagonistic to the pathogens of camphor-tree, 4 strains showed the effect of resolved inorganic phosphate, and 2 strains showed the effect of resolved organic phosphorus. Two strains had the ability of IAA generation, and 5 strains had different salt resistances. Considering the excellent traits, strain ZS-1 was identified as Microbacterium resistens and strain ZS-3 was identified as Bacillus megaterium. Both these strains could increase the height of camphor-tree.【Conclusion】This study reveals the species of endophytic bacteria in camphor-tree and establishes the basis for the biological control and promote the growth of camphor-tree. This study for the first time aimed to determine the underlying causes of diseases in urban greening through the screening of endophytic bacteria from healthy branches and leaves of camphor-tree in Shanghai.

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Memo

Memo:

收稿日期:2018-02-24 修回日期:2018-07-19
基金项目:上海市科技兴农重点攻关项目(沪农科攻字(2014)第5-6号); 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:陆蓝翔(382433922@qq.com)。*通信作者:叶建仁(jrye@njfu.com.cn),教授。

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Last Update: 2018-11-30