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无花果栽培品种遗传多样性分析及SSR指纹图谱构建(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2018年06期
Page:
197-202
Column:
研究简报
publishdate:
2018-12-15

Article Info:/Info

Title:
Genetic diversity analysis and fingerprint construction of fig(Ficus carica Linn.)cultivars by SSR markers
Author(s):
SUN Yanxiao1 WANG Xianrong1* SUN Lei2 JIA Ming2
(1. College of Biological and Environmental, Nanjing Forestry University, Nanjing 210037, China; 2. Shandong Academy of Forestry,Ji’nan 250014, China)
Keywords:
Ficus carica Linn.(fig) simple sequence repeat(SSR) fingerprint genetic diversity
Classification number :
S663.3
DOI:
10.3969/j.issn.1000-2006.201612028
Document Code:
A
Abstract:
【Objective】In recent years, with numbers of new fig(Ficus caria Linn.)cultivars have been introduced in China, it is necessary to classify and identify fig cultivars from the DNA molecular level. 【Method】We analyzed the genetic diversity and constructed DNA fingerprint of 30 fig cultivars by simple sequence repeat(SSR)molecular marker technology, which provided the scientific basis for the classification and identification of fig cultivars.【Results】The 24 fragments were generated from 7 pairs of SSR primers. The average of Na, Ne, and Shannon information index(I)were 3.43, 3.025 6, and 1.126 6, respectively. The polymorphic information content(PIC)values ranged from 0.682 8 to 0.892 5, with a mean of 0.816 4. The genetic similarity coefficient ranged from 0.291 7 to 1.000 0, and the average similarity coefficient was 0.629 4, indicating differences between the 30 fig cultivars. And thirty fig cultivars were divided into four categories when the genetic similarity coefficient was 0.59.【Conclusion】This fingerprinting experiment based on the SSR molecular markers distinguished similar cultivars and provided scientific basis for breeding and identification of new fig cultivars.

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Last Update: 2018-11-30