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|Table of Contents|

白桦BpTCP7基因启动子的克隆及表达分析(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2019年01期
Page:
32-38
Column:
研究论文
publishdate:
2019-01-28

Article Info:/Info

Title:
Cloning and expression analysis of BpTCP7 promoter from Betula platyphylla
Article ID:
1000-2006(2019)01-0032-07
Author(s):
REN LiDONG JingxiangYANG YangHUANG HaijiaoLI Huiyu*
Key Laboratory of Tree Genetic Imporement and Biotechnology, Northeast Forestry University,Harbin 150040,China
Keywords:
Betula platyphylla BpTCP7 promoter cis-elements analysis clone expression analysis
Classification number :
S722
DOI:
10.3969/j.issn.1000-2006.20186025
Document Code:
A
Abstract:
【Objective】BpTCP7 gene, a member of the CIN branch of TCP family, plays an important role in plant growth and development. In order to characterize the function of BpTCP7. 【Method】 A 1 791 bp flanking sequence upstream of the translation initiation codon was cloned, and putative cis-regulatory elements were deciphered from the promoter sequence of BpTCP7 using PLACE and Plant CARE web tools, and transferred into Arabidopsis thaliana. Then, the expression patterns were investigated during salt and drought stress. 【Result】The results showed that cis-regulatory elements involved in cell cycle, flowering, leaf development, multiple hormone-responsiveness, and stress response were included in the promoter region. The Gus reporter gene that was drived the BpTCP7 promoter was detected in all tissues and organs during the reproductive and vegetative phases. Notably, the BpTCP7 promoter was expressed in the leaf margin of young and mature leaves. The expression level of BpTCP7 gene was increased in transgenic A. thaliana after NaCl and polyethylene glycol(PEG)stresses compared with the control.【Conclusion】In conclusion, BpTCP7 participates in biological processes of leaf development, hormone response, drought and salt stress response, and to some extent regulates the development of all tissues and organs during the reproductive and vegetative growth phases.

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Last Update: 2019-01-28