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一株分离自黑翅土白蚁的粘质沙雷氏菌的鉴定及特性(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2019年01期
Page:
76-82
Column:
研究论文
publishdate:
2019-01-28

Article Info:/Info

Title:
Identification and characteristics of a strain of Serratia marcescens isolated from the termites,Odontotermes formosanus
Article ID:
1000-2006(2019)01-0076-07
Author(s):
FU Renjie QI Xuelian FENG Kai XIA XinruiTANG Fang*
Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China
Keywords:
termite physiological and biochemical characters 16S rDNA Serratia marcescens prodigiosin
Classification number :
S718;TV698.2+36
DOI:
10.3969/j.issn.1000-2006.201804003
Document Code:
A
Abstract:
【Objective】 With increasing attention on the ecological environment, biological control of termites needs to be developed.【Method】A strain of a bacteria producing red pigment was screened from Odontotermes formosanus. After being separated and purified, a purer strain was obtained and named SM1. Then, SM1 was inoculated into O. formosanus, which caused the healthy termites to turn red and die. The bacteria was identified based on its morphological ch-aracteristics, physiological and biochemical characteristics, red pigment, and 16S rDNA. 【Result】The bacterial colony was round, easy to pick up, had slight odor, was smooth and sticky, and had a diameter of 1-3 mm and a middle vermilion that was not transparent. In addition, ten physiological and biochemical characteristics of the SM1 strain were measured. These results showed that the bacterium was Serratia marcescens. Furthermore, the size of its cloned 16S rDNA fragment was 1 418 bp, and the homology with Serratia marcescens was 99%. Therefore, the bacteria was determined to be Serratia marcescens. Further, a higher purity of red pigment could be obtained by ultrasonic-assisted acetone extraction, and the pigment was dissolved in ethanol solution and then analyzed by ultraviolet full wavelength scanning. The results showed that the pigment had an obvious absorption peak at 535 nm, which suggested that the red pigment was a prodigiosin. 【Conclusion】The bacterium screened from Odontotermes formosanus was Serratia marcescens, which created a theoretical foundation for the biological control of termites.

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Last Update: 2019-01-28