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|Table of Contents|

NaCl胁迫下红砂种子萌动期差异表达基因的转录组分析(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2019年03期
Page:
28-36
Column:
研究论文
publishdate:
2019-05-15

Article Info:/Info

Title:
Transcriptome analysis of differentially expressed genes in Reaumuria soongorica seeds germination under NaCl stress
Article ID:
1000-2006(2019)03-0028-9
Author(s):
LIU Xiaowei1YANG Xiuyan1WU Haiwen1LIU Xiaoyan2ZHU Jianfeng1*ZHANG Huaxin1*
1.Research Center of Saline and Alkali Land of State Forestry and Grassland Administration, Beijing 100091, China; 2.College of Life Sciences of Henan Agricultural University, Zhengzhou 450000, China
Keywords:
Reaumuria soongorica NaCl stress seed germination differentially expressed genes transcriptome analysis
Classification number :
Q943.2
DOI:
10.3969/j.issn.1000-2006.201811020
Document Code:
A
Abstract:
【Objective】Screen differentially expressed genes(DEG)at the germination stage of Reaumuria soongorica under NaCl stress and provide genetic resources for the breeding of salt-tolerant species.【Method】The R. soongorica seeds were treated with pre-fitting germination threshold concentration(273 mmol/L, marked as M), optimum germination concentration(43 mmol/L, marked as L)and distilled water(marked as C), and each treatment was repeated three times. The seeds were taken out when the radicle broke through the seed coat but not more than 2 mm, and the transcriptome sequencing was performed.【Result】There were 210, 2 273 and 2 888 DEG screened out in comparison group of L and C(LvsC), M and C(MvsC), M and L(MvsL), respectively, of which 116 were up-regulated and 94 were down-regulated in LvsC, 1 781 were up-regulated and 492 were down-regulated in MvsC, and 2 165 were up-regulated and 723 were down-regulated in MvsL. These up-regulated genes are mainly enriched in ribosome, carbon metabolism, cytochrome P450 metabolism, glutathione metabolism and other pathways in KEGG, down-regulated genes are mainly enriched in sucrose and starch metabolism, endoplasmic reticulum protein processing and other processes. 【Conclusion】Under stress conditions, the DEG induce related reactions synergistic responses, which ultimately lengthen the hypocotyl cells and break through the seed coat to complete germination.

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Last Update: 2019-05-15