我们的网站为什么显示成这样?

可能因为您的浏览器不支持样式,您可以更新您的浏览器到最新版本,以获取对此功能的支持,访问下面的网站,获取关于浏览器的信息:

|Table of Contents|

蓝莓果实多酚提取物的抗炎活性研究(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2019年03期
Page:
152-156
Column:
专题报道
publishdate:
2019-05-15

Article Info:/Info

Title:
Anti-inflammatory activity of polyphenol extracts from blueberry fruit
Article ID:
1000-2006(2019)03-0152-05
Author(s):
YAO Bei1 ZHAO Huifang1 WU Wenlong1* LI Weilin12*
1. Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China; 2. Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China
Keywords:
blueberry polyphenols anti-inflammatory activity iNOS gene COX-2 gene
Classification number :
S663.2
DOI:
10.3969/j.issn.1000-2006.201805013
Document Code:
A
Abstract:
【Objective】 This study was performed to reveal the anti-inflammatory activity and mechanism of polyphenol extracts from blueberry fruit. 【Method】 The macrophage model was used to investigate the effects of polyphenols extracted from blueberry fruit on the inducible nitric oxide synthase(iNOS)gene and the cyclooxygenase-2(COX-2)gene expression and the NO secretion. 【Result】Blueberry polyphenol extracts significantly decreased NO secretion of lipopolysaccharide(LPS)-induced RAW264.7 macrophages, and higher concentrations of the polyphenol extract resulted in lower NO secretion. Blueberry polyphenol extracts lowered the expression of iNOS and COX-2 genes. The anti-inflammatory activity of polyphenol extracts from five blueberry cultivars was different in this test.【Conclusion】Blueberry polyphenol extract has anti-inflammatory activity by reducing NO secretion and inhibiting the expression of iNOS and COX-2 genes, thus alleviating the occurrence and development of the inflammation of LPS-induced RAW264.7 macrophages.

References

[1] 中国植物志编辑委员会. 中国植物志:57卷[M]. 北京: 科学出版社, 1991: 75-164.
Chinese Ethnographic Editorial Board. Chinese flora. Volume 57[M].Beijing: Science Newspaper, 1991: 75-164.
[2] KADER F, ROVEL B, GIRARDIN M, et al. Fractionation and identification of the phenolic compounds of Highbush blueberries(Vaccinium corymbosum L.)[J]. Food Chemistry, 1996, 55(1): 35-40.DOI: 10.1016/0308-8146(95)00068-2.
[3] OHSHIMA H, TAZAWA H, SYLLA B S, et al. Prevention of human cancer by modulation of chronic inflammatory processes[J]. Mutation Research/Fundamental & Molecular Mechanisms of Mutagenesis, 2005, 591(1-2): 110-122. DOI: 10.1016/j.mrfmmm.2005.03.030.
[4] LJUNG T, LUNDBERG S, VARSANYI M, et al. Rectal nitric oxide as biomarker in the treatment of inflammatory bowel disease: Responders versus nonresponders[J]. World Journal of Gastroenterology, 2006, 12(21): 3386. DOI: 1011111/j.1305-2036.2006.07295x.
[5] 章丹丹, 潘一峰, 唐宁, 等. 刘寄奴抗炎组分筛选及其机制研究[J]. 上海中医药杂志, 2009, 43(7): 67-71.
ZHANG D D, PAN Y F, TANG N,et al. Study on active constitutes of Artemisia anomala S Moore and its anti-inflammation mechanism in stimulated macrophages[J]. Shanghai Journal of Traditional Chinese Medicine, 2009, 43(7): 67-71.
[6] LALA P K, CHAKRABORTY C. Role of nitric oxide in carcinogenesis and tumour progression[J]. Lancet Oncology, 2001, 2(3): 149-156. DOI: 10.1016/s1470-2045100700256-4.
[7] BIAN K, MURAD F. Nitric oxide(NO)-biogeneration, regulation, and relevance to human diseases[J]. Frontiers in Bioscience A Journal & Virtual Library, 2003, 8(1): 264.DOI:10.1016/j.biocel.2006.03.04.
[8] TSATSANIS C, ANDROULIDAKI A, VENIHAKI M, et al. Signalling networks regulating cyclooxygenase-2[J]. International Journal of Biochemistry & Cell Biology, 2006, 38(10): 654.
[9] 李立萍, 张建新, 李兰芳, 等. 氨基胍对内毒素性肺损伤细胞凋亡的影响[J]. 中国药理学通报, 2007, 23(1): 28-32. DOI: 10.3321/j.issn:1001-1978.2007.01.009.
LI L P, ZHANG J X, LI L F,et al. Effect of aminoguanidine on pulmonary apoptosis in the lipopolysaccharide-induced acute lung injury in rats[J]. Chinese Pharmacological Bulletin, 2007, 23(1): 28-32.
[10] KOU X, QI S, DAI W, et al. Arctigenin inhibits lipopolysaccharide-induced iNOS expressionin RAW264.7 cells through suppressing JAK-STAT signal pathway[J]. International Immunopharmacology, 2011, 11(8): 1095-1102. DOI: 10.1016/j.intimp.2011.03.005.
[11] SHEEBA M S, ASHA V V. Cardiospermum halicacabum ethanol extract inhibits LPS induced COX-2, TNF-alpha and iNOS expression, which is mediated by NF-kappaB regulation, in RAW264.7 cells[J]. Journal of Ethnopharmacology, 2009, 124(1): 39-44.DOI:10.1016/j.jep.2009.04.020.
[12] LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T))method[J]. Methods, 2001, 25(4): 402-408.
[13] 李晓红, 齐云, 蔡润兰, 等. 芦荟大黄素对LPS诱导的RAW264.7细胞NO生成及iNOS表达的影响[J]. 中国药理学通报, 2010, 26(4): 488-492.
LI X H, QI Y, CAI R L,et al. Effect of lipopolysaccharide-induced expression of inducible nitric oxide synthase by aloe-emodin in RAW264.7 cells[J]. Chinese Pharmacological Bulletin, 2010, 26(4):488-492.
[14] 韩彩静, 王文亮, 陈相艳, 等. 蓝莓多酚对小鼠巨噬细胞中NO、iNOS及COX-2分泌量的影响[J]. 中国食品学报, 2015, 15(2):15-20. DOI: 10.16429/j.1009-7848.2015.02.003.
HAN C J, WANG W L, CHEN X Y, et al. Effects of the blueberry polyphenols on the secretion of NO, iNOS,COX-2 in mice macrophages[J]. Journal of Chinese Institute of Food Science and Technology, 2015, 15(2): 15-20.
[15] 宋盛, 邢达, 周非凡. PDT诱导的凋亡细胞对巨噬细胞NO合成的影响[J]. 生物物理学报, 2009, 17(s1):711-715. DOI: 10.3969/j.issn.1007-7146.2008.06.001.
SONG S, XING D, ZHOU F F. Effects of PDT-treated apoptotic cells on NO synthesized in macrophages[J]. Acta Biophysica Sinica, 2009, 17(s1):711-715.
[16] 罗剑刚, 李慧, 杨聪娴. COX-2选择性抑制剂治疗疼痛的研究进展[J]. 中国疼痛医学杂志, 2012, 18(2):66-69. DOI: 10.3969/j.issn.1006-9852.2012.02.002.
LUO J G, LI H, YANG C X. Progress in the treatment of pain by COX-2 selective inhibitors[J]. Chinese Journal of Pain Medicine, 2012, 18(2):66-69.
[17] CHENG A, YAN H, HAN C, et al. Polyphenols from blueberries modulate inflammation cytokines in LPS-induced RAW264.7 macrophages.[J]. International Journal of Biological Macromolecules, 2014, 69(8): 382-387. DOI: 10.1016/j.ijbiomac.2014.05.071.

Last Update: 2019-05-15