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Effect of cold acclimation on freezing tolerance and antioxidant enzyme activities of callus of Tibet Saussurea laniceps Hand.-Mazz
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SHE Lulu1 WANG Weijuan2 SONG Jingjing3 LU Cunfu1 CHEN Yuzhen1*
(1. The Key Laboratory of Educatin Ministry for Genetic Breeding and Gene Engineering of Woody and Ornamental Plants, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing 100083, China; 2. Rural Development Center of Beijing Municipal Commission of Science and Technology, Beijing 100195, China; 3. Key Enterprise Laboratory for Large-Scale Medicinal Plant Cell Culture, Tianjin Acelbio Co.Ltd., Tianjin 300457, China)
Saussurea laniceps callus cold-hardening freezing tolerance antioxidant enzyme antioxidant substance
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【Objective】 This study aimed to elucidate the mechanisms of physiological and biochemical adaptations of alpine plants to cold acclimation. 【Method】 The callus of Tibet Saussurea laniceps Hand.-Mazz. was selected as the plant material to assess changes in antioxidant enzymes and antioxidants during the process of cold acclimation(4 ℃/0 ℃, day/night, dark, for 0, 3, 6, 9, 12 days, respectively). 【Result】 During the period of cold acclimation, freezing tolerance peaked on day 9, with the lethal temperature(LT50)from -3.5 ℃ to -12.0 ℃. After 4 ℃/0 ℃ cold acclimation, soluble protein, proline content and antioxidant enzyme activity increased significantly, reaching maximum levels on day 9, on which protein content increased by 89.1%, proline content was increased 2.14-fold, and superoxide dismutase(SOD), catalase(CAT)and peroxidase(POD)activity increased by 48.04%, 47.28% and 73.18%, respectively. Furthermore, these results were confirmed by three antioxidant enzyme isozyme zymograms. Cold acclimation increased the content of polyphenols, which were increased 3.91-fold compared with the control on day 12. The polyphenol oxidase(PPO)activity increased by 91.35% compared with the control, and these results were consistent with those for the isozyme. 【Conclusion】 The freezing tolerance of S. laniceps was significantly increased after cold acclimation, showing a unique adaptation mechanism to the low temperature environment. This study established a feasible experimental system that is valid and referenced for studying endangered plants.


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Last Update: 2019-10-08