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赤霉素对‘大富贵’芍药休眠解除及内源激素和糖类代谢的影响(PDF/HTML)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2020年3期
Page:
26-32
Column:
专题报道 (执行主编 李维林)
publishdate:
2020-06-05

Article Info:/Info

Title:
Effects of GA3 on dormancy release, endogenous hormones levels and sugar metabolism in Paeonia lactiflora ‘Da Fugui’
Article ID:
1000-2006(2020)03-0026-07
Author(s):
JIANG Nannan12 ZHANG Qixiang1 WANG Yuan3 SUN Yin2 FANG Yifu2 XU Jinguang2
(1. Beijing Key Laboratory of Ornamental Plants Germplasm Innovation & Molecular Breeding, National Engineering Research Center for Floriculture, College of Landscape Architecture of Beijing Forestry University, Beijing, 100083, China ; 2. Shandong Academy of Forestry, Jinan 250014, China ; 3. Forest Seedling and Flower Station of Shandong Province, Jinan 250014, China )
Keywords:
gibberellin dormancy release sugar metabolism endogenous hormones
Classification number :
S682.1
DOI:
10.3969/j.issn.1000-2006.201909019
Document Code:
A
Abstract:
Objective We investigated the effects of gibberellin acid (GA3) (0, 100, 200, 300 and 1 000 mg/L) on dormancy release to replace low-temperature treatments; furthermore, physiological changes in endogenous hormones and carbohydrate metabolism were examined. The results may provide theoretical and technical support for applied use of GA3 to terminate dormancy and regulate flowering time in Paeonia lactiflora. Method We investigated effects of GA3 at different concentrations on plant growth and flowering using open-field overwintered potted P. lactiflora ‘Da Fugui’ as a control. We measured fructose, glucose and sucrose concentrations using high performance liquid chromatography (HPLC). Activities of enzymes associated with the metabolism of five sugar compounds and content of five endogenous hormones were determined using enzyme-linked immunoassays (ELISA) and liquid chromatography-mass spectrometry(LC-MS), respectively. Result The results showed that 100, 200, 300 and 1 000 mg/L GA3 affected dormancy termination in P. lactiflora ‘Da Fugui’, and substantial differences in plant growth and development were observed in treated seedlings. Stem lodging and wilting rates of seedlings treated with 100, 200, 300 and 1 000 mg/L GA3 were 0.88%, 18.1%, 65.7% and 100%, respectively. Treatments with 100-300 mg/L GA3 led to substantially decreased height (variation rate 17.9%-23.5%), number of flowers (variation rate 46.6%-65.9%), and flower diameter (variation rate 11.8%-16.7%); however, the flowering process was normal. During dormancy and during the bud scale bursting period, fructose and glucose were the predominant soluble sugars in ‘Da Fugui’ plants, and the proportion of sucrose increased du?ring bud swelling. During dormancy, bud swelling, and bud scale bursting, sucrose synthase, cell wall invertase, and vacuolar invertase activity increased, whereas sucrose phosphate synthase and alpha-amylase decreased slightly initially and then increased. Gibberellin, auxin, abscisic acid and salicylic acid content initially decreased and then increased, whereas jasmonic acid content showed the opposite pattern. Conclusion Treatments with 100-400 mg/L GA3 affected dormancy release in ‘Da Fugui’ plants, and the strongest effect was observed at 100 mg/L GA3 which produced the best growth and flowering results. Fructose and glucose are the main soluble sugars during bud dormancy and bud scale burs?ting, and nutrients produced in the roots are transferred to buds in form of sucrose during bud swelling. GA3 treatments induced considerable changes in the levels of five endogenous hormones, and subsequent synergistic effects elicited the transition from dormancy to seedling growth.

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Last Update: 2020-06-11