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矢竹地下茎节间生长的解剖学和转录组研究(PDF/HTML)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2020年3期
Page:
33-40
Column:
研究论文
publishdate:
2020-06-05

Article Info:/Info

Title:
Anatomical and transcriptomic analysis of bamboo rhizome internode growth
Article ID:
1000-2006(2020)03-0033-08
Author(s):
XIANG Yu DING Yulong ZHANG Chunxia WEI Qiang
(Co?Innovation Center for the Sustainable Forestry in Southern China, Bamboo Research Institute, Nanjing Forestry University, Nanjing210037, China)
Keywords:
internode growth fast growth RNA?Seq reactive oxygen species(ROS) cell wall rhizome
Classification number :
S795;Q781
DOI:
10.3969/j.issn.1000-2006.201906033
Document Code:
A
Abstract:
Objective The underground rhizome is the main stem of the running bamboo forest. However, little is known about its development, such as its elongation that is important for bamboo forest spread. In the present study, we aim to explore the cellular and transcriptomic features of rhizome internode elongation of Pseudosasa japonicas. Method Cellular observation was completed via paraffin sectioning, analysis of the transcriptomic profile via next?generation sequencing technology, and visualization, as well as enrichment analysis of differentially expressed genes, via MapMan. Result Through the morphological analysis of P. japonica’s rhizome elongation, we discovered that the elongation was directly promoted by the growth of -14 young internodes in the rhizome shoot. The anatomical analysis results further revealed that internodes with lengths lower than 0.4 cm had strong cell division ability, and distinctive cell elongation was found in a 1.0 cm long internode. Long and short parenchymal cells were apparent in the 1.0 cm internode and its vascular tissue such as vessel cells and fiber cells were much longer and more developed than in the 0.4 cm internode. It was also observed that pith tissue broke to form the pith cavity in the 1.0 cm internode. The RNA?Seq analysis between 0.4 and 1.0 cm internodes revealed a transition of transcriptomic activities. This included a transition from cell division and basic cellular metabolism in the 0.4 cm internode to active cell growth processes such as cell wall biosynthesis and secondary metabolism, and in the 1.0 cm internode to Programmed Cell Death processes such as ethylene signal pathways and (reactive oxygen species, ROS) burst genes. Conclusion There is a transition of cell growth processes from cell division to cell elongation during P. japonica’s rhizome internode elongation. Additionally, developmental events such as pith cavity formation exist in the 1.0 cm internode, which might be due to upregulation of genes related to cell growth and Programmed Cell Death.

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Last Update: 2020-06-11