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|Table of Contents|

TabZIP基因白桦的获得及耐盐性分析(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2013年05期
Page:
6-12
Column:
研究论文
publishdate:
2013-09-30

Article Info:/Info

Title:
TabZIP transferred Betula platyphylla generation and salt tolerance analysis
Article ID:
1000-2006(2013)05-0006-07
Author(s):
LI Yuanyuan1YANG Guang1WEI Rui1SUN Yanshuang1GUO Yuhua2 ZHANG Ruiping1LIU Guifeng1*
1. State Key Laboratory of Forest Genetics and Tree Breeding, Northeast Forestry University, Harbin 150040, China;
2. Heilongjiang College of Education,Harbin 150080, China
Keywords:
Betula platyphylla TabZIP gene physiological index salt tolerance
Classification number :
S71846
DOI:
10.3969/j.issn.1000-2006.2013.05.002
Document Code:
A
Abstract:
Plant basic leucine zipper (bZIP) proteins was an important group of plant transcription factors, as these proteins could interact with cisacting ABRE elements. These proteins activated the expression of some resistance related genes such as drought and salt tolerant genes. In order to create novel salttolerant transgenic lines, the transcription factor gene TabZIP was imported from Tamarix into the birch genome by using agrobacterium mediated transfection. Six transgenic lines were confirmed by qRTPCR and Western blotting. We compared the relative height growth rate, chlorophyll fluorescence parameters and salt injury index in transgenic lines before and after salt stress treatments. The results showed that the TB4 transgenic line was superior to other transgenic lines after salt stress treatments, with a relatively higher height growth(524%), increased by 73.5% if compared to control. The salt damage of TB4 transgenic gene was the lowest, with a salt injury index of 005, while controls were 019. After salt treatment, TB4 demonstrated different chlorophyll fluorescence parameters from the other transgenic lines, with the highest increase in the nonphotochemical quenching parameter, the lowest decrease in the actual photochemical efficiency of PSⅡ and the photochemical quenching coefficient, and the maximal quantum yield of PSⅡ. The changes in these indicators demonstrated that the expression of exogenous TabZIP could improve the resistance of plants to salt stress, and of these transgenic lines obtained would lay foundation for further research into the resistance mechanism of TabZIP.

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Last Update: 2013-09-30