我们的网站为什么显示成这样?

可能因为您的浏览器不支持样式,您可以更新您的浏览器到最新版本,以获取对此功能的支持,访问下面的网站,获取关于浏览器的信息:

|Table of Contents|

油茶带芽茎段及叶片离体培养再生体系的建立(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2013年05期
Page:
35-39
Column:
研究论文
publishdate:
2013-09-30

Article Info:/Info

Title:
Culture in vitro and rapid propagation techniques of buds and leafs in Camellia oleifera
Article ID:
1000-2006(2013)05-0035-05
Author(s):
YUAN Deyi FAN Xiaoming TAN Xiaofeng ZENG Yanling TANG Jing YANG Ya
The Key Lab of Nonwood Forest Nurturing and Protection of National Ministry of Education, Central South University of Forestry and Technology, Changsha 410004,China
Keywords:
Camellia olefera in vitro culture adventitious bud regeneration system
Classification number :
S722
DOI:
10.3969/j.issn.1000-2006.2013.05.007
Document Code:
A
Abstract:
The effects of plant growth regulators on the adventitious bud proliferation and rooting in Camellia oleifera were studied by using new shoot (with buds) and leaf as explants to establish a fast reproductive system. And the effects of different medium on survival rate of transplanted tissue culture seedlings were also investigated. The results showed that:(1) The highest frequency of shoot formation was obtained when explants (stem with buds) were cultured on 1/2MS+NAA(0.5 mg/L)+6-BA(1.0 mg/L)+ZT(0.5 mg/L). The inducement rate of valid buds reached to 745%. The regenerated shoot from leaf was obtained when cultured on 1/2MS+TDZ (1.0 mg/L)+IAA(0.1 mg/L)+6-BA(2.0 mg/L) for 7 weeks. (2) Regenerated shoot were cut as several cluster or single plantlet and transferred on 1/2MS+6-BA(1.0 mg/L)+NAA(0.05 mg/L) for shoot propagation and growth, which the multiplication coefficient was more than 417. (3) 883% of rooting rate, 372 cm of average root length, and 8 roots of average plantlet were observed when cultured in 1/2 MS for 50 days, after shoot was dipped in 1 g/L IBA for 5 second. (4) The rooted plantlets were acclimatized on mixture of peat and vermiculite(1∶1), which survival rate was 82.5%.

References

[1]胡芳名,谭晓风,刘惠明.中国主要经济树种栽培与利用[M].北京:中国林业出版社,2006.
[2]张日清,丁植磊,张勋,等.油茶育种研究进展[J].经济林研究,2006, 24(4): 1-8. Zhang R Q, Ding Z L, Zhang X, et al. Literature review of genetic improvement in oiltea [J]. Nonwood Forest Research, 2006, 24(4): 1-8.
[3]颜慕勤,陈平,韦民,等.油茶组织培养与大田栽培[J].林业科学, 1984, 20(4): 341-352. Yan M R, Chen P, Wei M, et al. Tissue culture and field cultivation of Camellia oleifera [J]. Scientia Silvae Sinicae, 1984, 20(4): 341-352.
[4]毕方诚,谭晓风,张智俊,等.油茶离体培养诱导再生植株的研究[J].经济林研究,2004, 22(2): 5-9. Bi F J, Tan X F, Zhang Z J, et al. Study on organogenesis and induction of regeneration plant of Camellia oleifera [J]. Nonwood Forest Research, 2004, 22(2): 5-9.
[5]吴幼媚,王以红,蔡玲,等.油茶单芽组培生根研究[J].西部林业科学,2012, 41(4): 25-28. Wu Y M, Wang Y H, Cai L, et al. Study on rooting of single buds of Camellia oleifera[J]. Journal of West China Forestry Science, 2012, 41(4): 25-28.
[6]王瑞,陈永忠,王湘南,等.油茶优良无性系叶片愈伤组织诱导研究[J].经济林研究,2009, 27(2): 35-39. Wang R, Chen Y Z, Wang X N, et al. Study on leaf callus induction of Camellia oleifera superior clone[J]. Nonwood Forest Research, 2009, 27(2): 35-39.
[7]范晓明,袁德义,谭晓风,等.油茶组织培养技术研究[D].长沙:中南林业科技大学,2011, 6. Fan X M, Yuan D Y, Tan X F, et al. Study on tissue culture of Camellia oleifera [D]. Changsha: Central South University of Forestry and Technology, 2011, 6.
[8]宋建英.邓恩桉的组织培养和植株再生[J].林业科学,2010, 46(6): 138-145. Song J Y. Bud micropropagation and plantlet-transplanting techniques for breeding coldresistant strain of Eucalyptus dunnii[J].Scientia Silvae Sinicae, 2010, 46(6): 138-145.
[9]Olaya P T, Jose E, Alicia V, et al. Assessment of factors affecting adventitious shoot regeneration from in vitro cultured leaves of apricot[J]. Plant Science,2000,158(1): 61-70.
[10]Sujatha M, Makkar H P S, Becher K. Shoot bud proliferation from axillary node and leaf of nontoxic Jatropha curcas L.[J]. Plant Growth Regulation, 2005, 47(1): 83-90.
[11]何月秋,叶建仁.湿地松丛生芽的诱导及试管苗的生根研究[J].南京林业大学学报,2005, 29(5): 13-16. He Y Q, Ye J R. Studies on the Axillary buds induction an plantlet rooting of Pinus elliottii[J]. Journal of Nanjing Forestry University:Natural Sciences Edition,2005, 29(5): 13-16.
[12]Carla R, Krystyna K, Mario R C, et al. Adventitious rooting of conifers: influence of physical and chemical factors[J]. Trees, 2010, 24(6): 975-992.
[13]张倩,王华芳.牡丹试管苗生根与移栽技术研究进展[J].园艺学报,2012, 39(9): 1819-1828. Zhang Q, Wang H F. In vitro shoot rooting and plantlet transplanting of tree peonies[J]. Acta Horticulturae Sinica, 2012, 39(9): 1819-1828.
[14]George E F, Sherrington. Plant Propagation by Tissue Culture[M]. Edington, Westbury, Wiltshire, England: Exegetics Ltd,1984.
[15]Chen J T, Chang W C. Efficient plant regeneration through somatic embryogenesis from callus cultures of Oncidium (Orchidaceae)[J]. Plant Science, 2000, 160(1): 87-93.

Last Update: 2013-09-30