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青钱柳叶多糖不同组分体外降血糖及抗氧化活性研究(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2017年04期
Page:
6-12
Column:
专题报道
column:
2017-07-31

Article Info:/Info

Title:
Hypoglycemic and antioxidant activity of various polysaccharides from Cyclocarya paliurus leaves in vitro
Article ID:
1000-2006(2017)04-0006-07
Author:
徐静珠1吴彩娥12*应瑞峰1李婷婷1范龚健1方升佐23
1.南京林业大学轻工科学与工程学院,江苏 南京 210037;
2.南方现代林业协同创新中心, 江苏 南京 210037;
3.南京林业大学林学院,江苏 南京 210037
Author(s):
XU Jingzhu1 WU Caie12* YING Ruifeng1 LI Tingting1 FAN Gongjian1FANG Shengzuo23
1.College of Light Industry Science and Engineering, Nanjing Forestry University, Nanjing 210037, China;
2.Co-Innovation Centre for Sustainable Forestry in Southern China, Nanjing 210037, China;
3.College of Forestry, Nanjing Forestry University, Nanjing 210037, China
Keywords:
青钱柳 多糖 降血糖 抗氧化
Keywords:
Cyclocarya paliurus polysaccharide hypoglycemic effect antioxidant activity
Classification number :
TS 201.4; S713
DOI:
10.3969/j.issn.1000-2006.2017.04.002
Document Code:
A
Abstract:
【目的】研究青钱柳叶多糖的体外降血糖及抗氧化活性。【方法】以对α-葡萄糖苷酶的抑制作用表示青钱柳叶多糖体外降血糖活性,以总抗氧化能力、清除DPPH自由基、羟基自由基能力和Fe2+螯合能力评价其体外抗氧化活性。【结果】青钱柳叶多糖各组分对α-葡萄糖苷酶均有一定的抑制作用,且呈现一定的剂量-效应关系。不同醇沉多糖组分中,50%醇沉组分对α-葡萄糖苷酶的抑制作用最强; 青钱柳叶多糖各组分均具有一定的抗氧化活性,不同醇沉组分清除DPPH自由基能力以及总抗氧化能力由强到弱依次为50% 组分、60% 组分、70%组分、80%组分,而清除羟基自由基能力以及对Fe2+螯合能力的强弱正好相反。【结论】青钱柳叶中含有的多糖组分具有良好的体外降血糖和抗氧化活性,可进一步加工利用。
Abstract:
【Objective】In order to evaluate the hypoglycemic and antioxidant activity of polysaccharides from Cyclocarya paliurus leaves in vitro. The activity of refined polysaccharides and precipitation fractionations of purified C. paliurus polysaccharide obtained from different concentrations of ethanol were compared in this study.【Method】The activity of inhibiting α-glucosidase was used to evaluate the hypoglycemic effect in vitro. The ability of total antioxidant, scavenging DPPH and(·OH)radicals and chelating Fe2+were used to evaluate the antioxidant capacity.【Result】The results showed that refined polysaccharides of C. paliurus and different precipitation fractionations all showed a high inhibition against α-glucosidase, and showed a dose-effect relationship. With the ethanol concentration increasing, the fractions obtained showed a higher inhibition activity. Refined polysaccharides of C. paliurus and different precipitation fractionations all showed a good antioxidant activity. Among different precipitation fractionations, the abilities of scavenging DPPH and total antioxidant were increased with the ethanol concentration increasing. In contrast, the abilities of scavenging(·OH)radicals and chelating Fe2+ were decreased.【Conclusion】Different C. paliurus polysaccharides have good in vitro hypoglycemic and antioxidant activity, which can be used for further manufacture and expoliting.

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Memo

Memo:
收稿日期:2016-10-31 修回日期:2017-02-18
基金项目:国家自然科学基金项目(31270673); 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:徐静珠(1551289377@qq.com)。*通信作者:吴彩娥(sxwucaie@163.com),教授,博士。
引文格式:徐静珠,吴彩娥,应瑞峰,等. 青钱柳叶多糖不同组分体外降血糖及抗氧化活性研究[J]. 南京林业大学学报(自然科学版),2017,41(4):6-12.
Last Update: 1900-01-01