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Analysis of genetic diversity and construction of DNA fingerprinting of clones in Cryptomeria fortune(PDF)

Journal of Nanjing Forestry University(Natural Science Edition)[ISSN:1000-2006/CN:32-1161/S]

Issue:
2017 04
Page:
191-196
Column:
publishdate:
2017-07-31

Article Info:/Info

Title:
Analysis of genetic diversity and construction of DNA fingerprinting of clones in Cryptomeria fortune
Article ID:
1000-2006(2017)04-0191-06
Author(s):
LUO Peng1 CAO Yuting1 MO Jiaxing1 WENG Huaifeng2 SHI Jisen1 XU Jin1*
1. Co-Innovotion Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing, 210037, China;
2 State-owned Forest Farm of Fujian Yangmeiling, Xiapu 355100, China
Keywords:
SSR marker Cryptomeria fortunei clones genetic diversity DNA fingerprinting
Classification number :
S791.31
DOI:
10.3969/j.issn.1000-2006.201611006
Document Code:
A
Abstract:
【Objective】Molecular fingerprinting and genetic diversity analysis are important for protecting and managing superior germplasm resources, as well as for identifying superior clones. 【Method】In the present study, 11 SSR primer pairs were used to assess the genetic diversity and DNA fingerprints of 89 C. fortunei clones. 【Result】A total of 53 putative alleles were detected from 11 pairs of primers, with a mean of five putative alleles per locus, ranging from three to six. The number of effective alleles(Ne)ranged from 1.887 0 to 4.295 6, with a mean of 3.041 1, and the Shannon's information index ranged from 0.774 9 to 1.556 3, with a mean of 1.208 1. These findings indicate that SSR markers in C. fortunei exist a relative high polymorphism. Furthermore, according to the characteristics of the SSR markers and primer sequences, the present(recorded as 1)and absent(recorded as 0)collected from amplification by SSR markers were transferred into genetic types. By combining different genetic types code, we established DNA fingerprints of 89 C. fortunei clones, and attached a 22-digit fingerprint number to each clone. The 89 clones were also separated into five clusters based on genetic distance. 【Conclusion】SSR markers are suitable for analyzing and identifying the genetic diversity of C. fortune, and C. fortune shows moderate to high genetic diversity.

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