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61个观赏海棠品种的SSR指纹图谱构建及遗传多样性分析(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2018年03期
Page:
45-50
Column:
专题报道(Ⅱ)
publishdate:
2018-05-15

Article Info:/Info

Title:
The construction of fingerprints and genetic diversity analysis of 61 Malus crabapple cultivars based on SSR markers
Article ID:
1000-2006(2018)03-0045-06
Author(s):
RONG Hao HUANG Bin ZHOU Qi ZHANG Wangxiang XU Li'an*
Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037,China
Keywords:
Keywords:ornamental crabapple SSR marker fingerprint genetic diversity
Classification number :
S722
DOI:
10.3969/j.issn.1000-2006.201801004
Document Code:
A
Abstract:
【Objective】There are many kinds of Malus crabapple cultivars because of homonyms and synonyms. However, the classification and identification of phenotypic traits can no longer satisfy the classification of Malus crabapple cultivars. Therefore, it is particularly important to develop a rapid and accurate method of identifying Malus crabapple cultivars.【Methods】In this study, sixty-one major Malus crabapple cultivars were tested for PCR amplification using 10 pairs of SSR primers selected out from the Malus multi-species. 【Results】A total of 78 bands were identified from the 10 pairs of SSR primers. The average of bands per marker was 7.8 and the percentage of polymorphic bands was 100%. The Nei gene diversity(H)and Shannon index(I)of 61 Malus crabapple cultivars were 0.205 2 and 0.348 8, respectively. If analyzed as co-dominant markers, the Nei gene diversity of 43 Malus crabapple cultivars ranged from 0.623 9 to 0.881 8, with an average of 0.788 3, exhibiting a high level of genetic diversity.【Conclusion】In conclusion, the fingerprints of 61 ornamental crabapple cultivars were successfully constructed by using two pairs of SSR primers, which provided assistance in the identification and in-depth exploitation of cultivar resources

References

[1] 钱关泽, 汤庚国. 苹果属植物分类学研究进展[J]. 南京林业大学学报(自然科学版), 2005, 29(3): 94-98.DOI:10.3969/j.jssn.1000-2006.2005.03.024. QIAN G Z,TANG G G. A review on the plant taxonomic study on the genus Malus Miller[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2005, 29(3): 94-98.
[2] 李育农. 苹果属植物种质资源研究[M]. 北京:中国农业出版社, 2001.
[3] 郑杨, 曲晓玲, 郭翎,等. 观赏海棠资源谱系分析及育种研究进展 [J]. 山东农业大学学报(自然科学版), 2008, 39(1):152-160. DOI:10.3969/j.issn.1000-2324.2008.01.033. ZHENG Y, QU X L, GUO L, et al. Advances on ornamental crabapple resources [J]. Journal of Shandong Agricultural University(Natural Science), 2008, 39(1):152-160.
[4] 周玉珍, 李火根, 张燕梅, 等. 墨西哥落羽杉无性系RAPD指纹图谱的构建[J]. 南京林业大学学报(自然科学版), 2006, 30(5): 29-33.DOI:10.3969/j.jssn.1000-2006.2006.05.007. ZHOU Y Z,LI H G,ZHANG H Y,et al. RAPD fingerprints of Clones for Taxodium mucronatum Ten[J]. Journal of Nanjing Forestry Unive rsity(Natural Sciences Edition), 2006, 30(5): 29-33.
[5] 高华. 苹果栽培品种的DNA指纹图谱构建及遗传多样性分析[D]. 杨凌:西北农林科技大学, 2010. GAO H. DNA fingerprinting and genetic diversity in apple cultivars [D]. Yangling: Northwest A & F University,2010.
[6] 岳娜, 胡倩倩, 方星, 等. 枇杷种质资源SSR标记指纹图谱的构建[J]. 中国农学通报, 2012, 28(16): 186-189.DOI:10.3969/j.issn.1000-6850.2012.16.034. YUE N,HU Q Q,FANG X,et al. Fingerprinting patterns of SSR markers constructed for 33 loquat [Eriobotrya japonica(Thunb.)Lindl.] germplasms[J]. Chinese Agricultural Science Bulletin, 2012, 28(16): 186-189.
[7] 徐宗大, 赵兰勇, 张玲, 等. 玫瑰SRAP遗传多样性分析与品种指纹图谱构建[J]. 中国农业科学, 2011, 44(8): 1662-1669. DOI: 10.3864/j.issn.0578-1752.2011.08.015. XU Z D,ZHAO L Y,ZHANG L,et al. Analysis of genetic diversity and construction of fingerprint of Rosa rugosa by SRAP[J]. Scientia Agricultura Sinica, 2011, 44(8): 1662-1669.
[8] 王星星, 周琦, 陶园园, 等. 48个果用银杏品种SSR指纹图谱构建与遗传多样性分析[J]. 分子植物育种, 2017, 15(5): 1963-1970. DOI: 10.13271/j.mpb.015.001963. WANG X X,ZHOU Q,TAO Y Y,et al. The fingerprints construction and genetic diversity analysis of 48 fruitused ginkgo cultivars based on SSR Markers[J]. Molecular Plant Breeding, 2017, 15(5): 1963-1970.
[9] GREINER R, VOGT R, OBERPRIELER C. Evolution of the polyploid north-west Iberian Leucanthemum pluriflorum clan(Compositae, Anthemideae)based on plastid DNA sequence variation and AFLP fingerprinting[J]. Annals of Botany, 2013, 111(6): 1109. DOI: 10.1093/aob/mct075.
[10] GORJI A M, POCZAI P, POLGAR Z, et al. Efficiency of arbitrarily amplified dominant markers(SCOT, ISSR and RAPD)for diagnostic fingerprinting in tetraploid potato[J]. American Journal of Potato Research, 2011, 88(3): 226-237. DOI: 10.1007/s12230-011-9187-2.
[11] 陶爱芬, 魏嘉俊, 刘星, 等. 应用SRAP标记绘制88份南瓜属种质资源DNA指纹图谱[J]. 植物遗传资源学报, 2017, 18(2): 225-232. DOI: 10.13430 /j.cnki.jpgr.2017.02.008. TAO A F,WEI J J,LIU X,et al. Construction of molecular fingerprinting map for 88 accessions of cucurbita by SRAP markers[J]. Journal of Plant Genetic Resources, 2017, 18(2): 225-232.
[12] 杨晴, 杨菲, 杨康, 等. 秦皇岛产柽柳ISSR遗传多样性分析与指纹图谱构建[J]. 中草药, 2017, 48(2): 363-367. DOI: 10.7501/j.issn.0253-2670.2017.02.024. YANG Q,YANG F,YANG K,et al. Analysis of genetic diversity and construction of fingerprint on Tamarix chinensis from Qinhuangdao by ISSR[J]. Chinese Traditional and Herbal Drugs, 2017, 48(2): 363-367.
[13] 刘欢, 张新全, 马啸, 等. 基于荧光检测技术的多花黑麦草EST-SSR指纹图谱的构建[J]. 中国农业科学, 2017, 50(3): 437-450. DOI:10.3864/j.issn.0578-1752.2017.03.003. LIU H,ZHANG X Q,MA X,et al. Construction of EST-SSR fingerprinting based on fluorescence detection technology for Italian Ryegrass[J]. Scientia Agricultura Sinica,2017, 50(3): 437-450.DOI:10.3864/j.issn.0578-1752.2017.03.003.
[14] 姚玉新, 翟衡. 苹果基因组分子生物学研究进展[J]. 果树学报, 2004, 21(6): 586-591.DOI:10.3969/j.issn.1009-9980.2004.06.019. YAO Y X,ZHAI H. A review of molecular studies on apple genome[J]. Journal of Fruit Science, 2004, 21(6): 586-591.
[15] HOKANSON S C, LAMBOY W F, SZEWCMCFADDEN A K, et al. Microsatellite(SSR)variation in a collection of Malus(apple)species and hybrids[J]. Euphytica, 2001, 118(3): 281-294. DOI: 10.1023/A:1017591202215.
[16] L G, CM O. Molecular characterisation of cultivars of apple(Malus×domestica Borkh.)using microsatellite(SSR and ISSR)markers[J]. Euphytica, 2001, 122(1): 81-89.DOI:10.1023/A:1012691814643.
[17] LIEBHARD R, KOLLER B L, GESSLER C. Creating a saturated reference map for the apple(Malus × domestica Borkh.)genome[J]. Tag Theoretical & Applied Genetics, theoretische Und Angewandte Genetik, 2003, 106(8): 1497.DOI:10.1007/s00122-003-1209-0.
[18] MARTINS W, DE SOUSA D, PROITE K, et al. New softwares for automated microsatellite marker development [J]. Nucleic Acids Res, 2006, 34(4): e31. DOI:10.1093/nar/gnj030.
[19] 左力辉, 韩志校, 梁海永, 等. 不同产地中国李资源遗传多样性SSR分析 [J]. 园艺学报, 2015, 42(1): 111-118. DOI:10.16420/j.issn.0513-353x.2014-0679. ZUO L H, HAN Z X, LIANG H Y, et al. Analysis of genetic diversity of Prunus salicina from different producing areas by SSR markers [J]. Acta Horticulturae Sinica, 2015, 42(1): 111-118.
[20] YAO L, ZHENG X, CAI D, et al. Exploitation of Malus EST-SSRs and the utility in evaluation of genetic diversity in Malus and Pyrus [J]. Genetic Resources and Crop Evolution, 2010, 57(6): 841-851. DOI: 10.1007/s10722-009-9524-1.
[21] LIEBHARD R, GIANFRANCESCHI L, KOLLER B, et al. Development and characterisation of 140 new microsatellites in apple(Malus×domestica Borkh.)[J]. Molecular Breeding, 2002, 10(4): 217-241. DOI: 10.1023/A:1020525906332.
[22] SILFVERBERG-DILWORTH E, MATASCI C L, VAN De WEG W E, et al. Microsatellite markers spanning the apple(Malus×domestica Borkh.)genome [J]. Tree Genetics & Genomes, 2006, 2(4): 202-224. DOI:10.1007/s11295-006-0045-1.
[23] 王立新, 张小军, 史星雲, 等. 苹果栽培品种SSR指纹图谱的构建 [J]. 果树学报, 2012(6): 971-977. DOI:10.13925/j.cnki.gsxb.2012.06.009. WANG L X, ZHANG X J, SHI X Y, et al. Establishment of SSR fingerprinting database on major apple(Malus ×domestica)cultivars [J]. Journal of Fruit Science, 2012(6): 971-977.
[24] 沈红香, 赵天田, 宋婷婷,等. 观赏海棠‘王族'自然杂交后代的遗传多样性分析 [J]. 园艺学报, 2011, 38(11):2157-2168.DOI: 10.16420/j.issn.0513-353x.2011.11.017. SHEN H X, ZHAO T T, SONG T T, et al. Genetic diversity analysis in natural hybrid progeny of ornamental crabapple,Malus ‘Royalty' [J]. Acta Horticulturae Sinica, 2011, 38(11):2157-2168.
[25] 曹福亮, 黄敏仁, 桂仁意,等. 银杏主要栽培品种遗传多样性分析 [J]. 南京林业大学学报(自然科学版), 2005, 29(6):1-6.DOI: 10.3969/j.jssn.1000-2006.2005.06.001. CAO F L, HUANG M R, GUI R Y, et al.The tingerprinting and genetic diversity of main ginkgo cultivars [J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2005, 29(6):1-6.
[26] 高源, 刘凤之, 王昆, 等. 苹果部分种质资源分子身份证的构建[J]. 中国农业科学, 2015, 48(19): 3887-3898. DOI: 10.3864/j.issn.0578-1752.2015.19.011. GAO Y,LIU F Z,WNAG K,et al. Establishment of molecular ID for some apple germplasm resources[J]. Scientia Agricultura Sinica, 2015, 48(19): 3887-3898.
[27] 段一凡, 王贤荣, 梁丽丽, 等. 桂花品种SSR荧光指纹图谱的构建[J]. 南京林业大学学报(自然科学版), 2014, 38(S1): 1-6.DOI:10.3969/j.issn.1000-2006.2014.S1.001. DUAN Y F,WANG R,LIANG L L,et al. Fingerprinting and identification of Osmanthus fragrans cultivars using fluorescence-labeled SSR markers[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2014, 38(S1): 1-6.
[28] CANTINI C. DNA Fingerprinting of tetraploid cherry germplasm using simple sequence repeats[J]. Journal of the American Society for Horticulturalence, 2001, 126(2): 205-209

Last Update: 2018-06-06