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核桃V-ATPase c亚基基因(JrVHAc4)的克隆和抗旱功能分析(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2019年02期
Page:
79-85
Column:
研究论文
publishdate:
2019-03-30

Article Info:/Info

Title:
Drought resistance analysis of a V-ATPase c subunit(JrVHAc4)gene from Juglans regia
Article ID:
1000-2006(2019)02-0079-07
Author(s):
LI Dapei12WANG Yi 1ZHANG Shangkun 1ZHAO Xiang 12ZHAO Huanyuan 12LIU Yumei 12YANG Guiyan 12 *
(1. Walnut and Chestnut Experiment Station of Shanyang, College of Forestry, Northwest A & F University,Yangling 712100, China; 2. Key Laboratory of Economic Plant Resources Development and Utilization in Shaanxi Province, College of Forestry, Northwest A & F University, Yangling 712100, China)
Keywords:
Juglans regia drought stress V-ATPase expression analysis yeast expression system
Classification number :
Q786
DOI:
10.3969/j.issn.1000-2006.201811014
Document Code:
A
Abstract:
【Objective】 V-ATPase is an important enzyme in plant stress response. This study is under stunll the functional mechanism of V-ATPase subunits in response to stress in walnuts. 【Method】 A V-ATPase c subunit gene(named JrVHAc4)was cloned from the ‘Xiangling’ walnut transcriptome. The stress response function was predicted by analyzing its promoter and expression under drought stress. Meanwhile, JrVHAc4 was inserted into a yeast expression vector pYES2 and transformed into yeast INVSC1 to study its drought resistance function. 【Result】 The open reading frame(ORF)of JrVHAc4 was 495 bp in length, the deduced protein was 16 527.61 u and contained 164 amino acids, whose theoretical isoelectric point(pI)was 8.62. An upstream 1 047 bp promoter segment was identified and found to contain abundant cis-elements, among which some are related to drought response, such as MYC, DOF and MYB. JrVHAc4 was significantly induced in leaves and roots by drought stress, and the induction in leaves and roots were different. Compared with control yeast, we found that expression of JrVHAc4 increased the growth activity of transgenic yeast significantly under different concentrations of mannitol stress. 【Conclusion】 JrVHAc4 can respond to drought stress and improve the drought resistance of transgenic yeasts, JrVHAc4 can be used as an important candidate gene for walnut resistance.

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Last Update: 2019-03-30