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|Table of Contents|

无机氮素形态对银杏苗期碳氮代谢的影响(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2019年02期
Page:
197-202
Column:
研究简报
publishdate:
2019-03-30

Article Info:/Info

Title:
Nitrogen form and ratio affect the carbon-nitrogen metabolism of Ginkgo biloba
Article ID:
1000-2006(2019)02-0197-06
Author(s):
HUANG Dongjing CAO Fuliang WANG Guibin*
(Co-Innovation Center for the Sustainable Forestry in Southern China, College of Forestry,Nanjing Forestry University, Nanjing 210037, China)
Keywords:
Ginkgo biloba nitrate nitrogen ammonium nitrogen carbon-nitrogen metabolite
Classification number :
S792.95
DOI:
10.3969/j.issn.1000-2006.201807043
Document Code:
A
Abstract:
【Objective】The effects of nitrogen form and ratio on carbon-nitrogen metabolism and related enzyme activities in the leaves of Ginkgo biloba seedlings were investigated to provide a theoretical basis for N fertilizer management of Ginkgo biloba. 【Method】Using 1-year-old seedlings of Ginkgo biloba as test materials, a greenhouse pot experiment was performed with 5 ratios of m(NH4+-N):m(NO3-N), namely T1(100:0), T2(75:25), T3(50:50), T4(25:75), and T5(0:100). We analyzed the content of total nitrogen, soluble protein, free amino acid, total carbon, soluble sugar, starch and sucrose, and the activity of amylase(AMS), sucrose synthetase(SS), sucrose phosphate synthase(SPS), nitrate reductase(NR), glutamine synthetase(GS), and glutamine synthetase(GOGAT)in the Ginkgo biloba seedlings under different treatments. 【Result】The carbon-nitrogen metabolites(except soluble protein content)and related enzyme activities in the leaves of Ginkgo biloba increased initially and then decreased, with an increase in the NO3--N ratio. In terms of carbon-nitrogen metabolism, the content of sucrose and free amino acids were the highest in the T3 group, and the content of total nitrogen, total carbon, soluble sugar, soluble protein, starch, and sucrose were the highest in the T4 group. The content of carbon-nitrogen metabolism indicators, except for soluble protein content, were the lowest in the T1 group. In terms of enzyme activity, SS and SPS activities were the highest in the T3 group, and AMS, NR, GS and GOGAT activities were the highest in the T4 group. AMS, SS, and NR activities were the lowest in the T1 group, and SPS, GS and GOGAT activities were the lowest in the T5 group. Overall, the contents of carbon-nitrogen metabolites and related enzyme activities in T3 and T4 groups were significantly higher than that in other groups, but no significant differences were observed between the T3 and T4 groups. 【Conclusion】The effects of NH4+-N and NO3--N mixed supply on the carbon-nitrogen metabolism of Ginkgo biloba were significantly higher than those of the single supply. The effect of 25:75 m(NH4+-N):m(NO3--N)was the best, and appropriate adjustments should be performed on the basis of the conditions.

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Last Update: 2019-03-30