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|Table of Contents|

白桦BpTCP8基因生物信息学及表达特性分析(PDF)

《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

Issue:
2019年05期
Page:
67-73
Column:
研究论文
publishdate:
2019-09-20

Article Info:/Info

Title:
Bioinformatics and expression characteristics analysis of BpTCP8 in Betula platyphylla Suk.
Article ID:
1000-2006(2019)05-0067-07
Author(s):
AN Linjun LUAN Jiayu REN Li LI Huiyu XIA De’an*
(School of Forestry, Northeast Forestry University, Harbin 150040, China)
Keywords:
Betula platyphylla Suk. BpTCP8 gene expression characteristics hormone response stress response
Classification number :
S722
DOI:
10.3969/j.issn.1000-2006.201811066
Document Code:
A
Abstract:
【Objective】To reveal the function of BpTCP8 in the growth and development of Betula platyphylla Suk., we analyzed the sequence characteristics, expression pattern in different tissues and the BpTCP8 gene response to hormones and stress.【Method】The sequence characteristics of BpTCP8 gene were analyzed using bioinformatic methods. Besides, the expression characteristics of the BpTCP8 gene in different tissues as well as its response to hormones and stress were analyzed by real-time fluorescent quantitative PCR.【Result】Using bioinformatic tools, it was found that the predicted amino acid sequence for the BpTCP8 gene contained a highly conserved bHLH domain, similar to that of the TCP family of transcriptional regulators. Results from qRT-PCR analyses indicated that BpTCP8 was highly expressed during the early developmental stages and during leaf senescence; BpTCP8 was also found to be upregulated in leaves with deep-splitting, apical buds, the xylem and the phloem. Different expression patterns of BpTCP8 were exhibited under different hormone treatments(GA3, ME-JA and ABA)and abiotic stresses(high PEG, NaCl, CdCl2 and NaHCO3 concentrations).【Conclusion】Therefore, the BpTCP8 gene may be involved in leaf maturation and leaf shape, and in the growth of apical buds, the xylem, and the phloem of B. platyphylla Suk.. The gene is positively regulated by GA3 and JA. BpTCP8 expression may also affect plant resistance to drought, salt, alkali and heavy metals through the ABA signaling pathway.

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Last Update: 2019-10-08