[1]阮成江,钦佩*,韩睿明,等.AFLP分子标记在鉴定海滨锦葵杂交后代上的应用[J].南京林业大学学报(自然科学版),2005,29(01):020-24.[doi:10.3969/j.jssn.1000-2006.2005.01.005]
 RUAN Cheng-jiang,QIN Pei*,HAN Rui-ming,et al.Identification of Hybrids of Kosteletzkya virginica Using AFLP Markers[J].Journal of Nanjing Forestry University(Natural Science Edition),2005,29(01):020-24.[doi:10.3969/j.jssn.1000-2006.2005.01.005]
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AFLP分子标记在鉴定海滨锦葵杂交后代上的应用
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
29
期数:
2005年01期
页码:
020-24
栏目:
研究论文
出版日期:
2005-01-20

文章信息/Info

Title:
Identification of Hybrids of Kosteletzkya virginica Using AFLP Markers
文章编号:
1000-2006(2005)01-0020-05
作者:
阮成江12钦佩1*韩睿明1何祯祥1
1. 南京大学生命科学学院, 江苏 南京 210093; 2. 大连民族学院, 辽宁 大连 116600
Author(s):
RUAN Cheng-jiang12 QIN Pei1* HAN Rui-ming1 HE Zhen-xiang1
1. School of Life Science Nanjing University, Nanjing 210093, China; 2. Dalian Nationalities University, Dalian 116600, China
关键词:
海滨锦葵 AFLP 杂种 分子鉴定
Keywords:
Kosteletzkya virginica AFLP Hybrid Molecular identification
分类号:
S722.3;Q819
DOI:
10.3969/j.jssn.1000-2006.2005.01.005
文献标志码:
A
摘要:
<正>为了解喀斯特地区榆科树种对水分胁迫的反应和适应,比较其抗旱性,采用人工模拟水分胁迫的方法,研究了青檀、朴树、榔榆幼苗部分生理生化指标的变化。结果表明:水分胁迫条件下,榆科3种幼苗抗旱生理生化指标的变化趋势一致。随胁迫程度加剧,水分饱和亏缺增大,水势降低,质膜透性增大,膜伤害程度增加,渗透调节物质脯氨酸含量增加。膜脂过氧化产物MDA含量表现出“增加一下降—-再增加”的变化过程,但各指标的变化程度因树种不同而异。用坐标综合评定法对3个树种幼苗抗旱能力的初步评价结果表明,榔榆幼苗的综合抗旱能力最强,青檀次之,朴树较差。
Abstract:
Kosteletzkya virginica is a potential halophytic grain crop for consumption and industrial utilization. AFLP markers were used to identify 36 F1 hybrids of two parents. Eighteen primer combinations amplified 1 100 AFLP bands between parent Hyl03 and Hh182,108 (9. 82%) of which were polymorphic. Total 1 182 AFLP bands were amplified between parent Slhy353 and Blho301,148(12. 51%) of which were polymorphic. Primer combination E+AAC/M4-CTC amplified five paternal characteristic bands between Hyl03 and Hh182; 401 bp, 390 bp, 156 bp, 137 bp and 108 bp,whereas Eq-AAG/M+CTG generated seven paternal characteristic bands:457 bp,356 bp,312 bp,251 bp,240 bp,190 bp and 76 bp. All these paternal characteristic bands were found in eight F1 hybrids of Hy103 × Hh182,which were real hybrid. Primer combination E+AAC/M+CTT amplified five paternal characteristic bands between Slhy353 and Blho301:440 bp,393 bp,289 bp,162 bp and 67 bp,whereas Eq-ACA/M+CAT generated four characteristic bands.. 448 bp,360 bp,217 bp and 145 bp. All these paternal characteristic bands were found in 26 of 28 F1 hybrids of S1hy353 × Blho301,and other two F1 hybrids were false hybrids.

相似文献/References:

[1]聂小安,蒋剑春,高一苇,等.海滨锦葵油生物柴油的制备及性能分析[J].南京林业大学学报(自然科学版),2008,32(01):076.[doi:10.3969/j.jssn.1000-2006.2008.01.017]
 NIE Xiao-an,JIANG Jian-chun,GAO Yi-wei,et al.Preparation technique of biodiesel from Kosteletzktya virginica oil and analysis of their properties[J].Journal of Nanjing Forestry University(Natural Science Edition),2008,32(01):076.[doi:10.3969/j.jssn.1000-2006.2008.01.017]
[2]阮成江,何祯祥,钦佩,等.海滨锦葵数量性状的相关和主成分分析(英文)[J].南京林业大学学报(自然科学版),2003,27(05):006.[doi:10.3969/j.jssn.1000-2006.2003.05.002]
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更新日期/Last Update: 2013-05-20