[1]张大生,崔丽洁,王景明,等.红巴梨f3h基因的克隆及植物表达载体的构建[J].南京林业大学学报(自然科学版),2005,29(02):065-68.[doi:10.3969/j.jssn.1000-2006.2005.02.016]
 ZHANG Da-sheng,CUI Li-jie,WANG Jing-ming,et al.Cloning f3h Gene from "Red Ballet" Fruit and Its Construction of Plant Express Vector[J].Journal of Nanjing Forestry University(Natural Science Edition),2005,29(02):065-68.[doi:10.3969/j.jssn.1000-2006.2005.02.016]
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红巴梨f3h基因的克隆及植物表达载体的构建
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
29
期数:
2005年02期
页码:
065-68
栏目:
研究论文
出版日期:
2005-02-20

文章信息/Info

Title:
Cloning f3h Gene from "Red Ballet" Fruit and Its Construction of Plant Express Vector
文章编号:
1000-2006(2005)02-0065-04
作者:
张大生1崔丽洁2王景明1吴少华2*
1. 徐州师范大学生命科学学院, 江苏 徐州 221116; 2. 福建农林大学园艺学院, 福建 福州 350002
Author(s):
ZHANG Da-sheng1 CUI Li-jie2 WANG Jing-ming1 WU Shao-hua2*
1. School of Life Science, Xuzhou Normal University, Xuzhou 221116, China; 2. College of Horticulture, Fujian Agricultural & Forestry University, Fuzhou 350002, China
关键词:
花青素 f3h基因 克隆 载体
Keywords:
Anthocyanin f3h gene Clone Vector
分类号:
Q813
DOI:
10.3969/j.jssn.1000-2006.2005.02.016
文献标志码:
A
摘要:
<正>对}te[mholtz型脉动发动机的主体结构燃烧室一喷管进行了研究,分析了5种燃烧室柱部直径的发动机所构成的声学条件,以及3种油门开度所构成的加热条件对发动机工作频率的影响。结果表明,在燃烧室柱部直径变化范围内,声学条件对发动机工作频率的影响不大,加热条件则显著改变发动机的工作频率。
Abstract:
The fragment of f3h gene of anthocyanin biosynthesis was amplified by RT-PCR method using total RNA from ripping "Red Ballet" fruit peel as template and oligonucleotides designed from the published sequence of kernel fruits as primers. Sequence analysis showed that this fragment is full-length cDNA of f3h gene and it contained 1 095 base pairs. Sequence comparison revealed that the f3h gene of "Red Ballet" shared homologies of 92%, 83% and 82% with those of "Fuji" apple, Petunia and Arabidopsis isolates., respectively. Then the f3h gene was digested by EcoR V and Bgl Ⅱ endoenzymes and 1 095 bp fragment was recovered. Vector pBI121 and 1 095 bp fragment,were connected using ligase. Reconstituted plasmid pBI12l included the anticipated fragment of f3h gene was obtained and was proved by antibiotic selection and PCR analysis. The reconstituted plasmid is using for the further study.
更新日期/Last Update: 2013-05-20