[1]李相前,邵蔚蓝*.降解结晶纤维素极耐热葡聚糖酶重组菌的构建[J].南京林业大学学报(自然科学版),2006,30(03):037-40.[doi:10.3969/j.jssn.1000-2006.2006.03.007]
 LI Xiang-qian,SHAO Wei-lan*.Construction of Recombinant Escherichia coli for Producing Extremely Thermostability Endoglucanase[J].Journal of Nanjing Forestry University(Natural Science Edition),2006,30(03):037-40.[doi:10.3969/j.jssn.1000-2006.2006.03.007]
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降解结晶纤维素极耐热葡聚糖酶重组菌的构建
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
30
期数:
2006年03期
页码:
037-40
栏目:
研究论文
出版日期:
2006-03-20

文章信息/Info

Title:
Construction of Recombinant Escherichia coli for Producing Extremely Thermostability Endoglucanase
文章编号:
1000-2006(2006)03-0037-04
作者:
李相前12邵蔚蓝13*
1. 江南大学工业生物技术教育部重点实验室, 江苏 无锡 214036; 2. 淮阴工学院生物工程与化学工程系, 江苏 淮安 223001; 3. 南京师范大学生命科学学院, 江苏 南京 210097
Author(s):
LI Xiang-qian12 SHAO Wei-lan13*
1. The Key Laboratory of Industrial Biotechnology of Education Ministry Southern Yangtze University, Wuxi 214036, China; 2. Department of Chemical and Bioengineering Huaiyin Institute of Technology, Huai’an 223001, China; 3. School of Life Science Nanjing Normal University, Nanjing 210097, China
关键词:
结晶纤维素 基因融合 内切葡聚糖酶
Keywords:
Crystalline cellulose Gene fusion Thermostability endoglucanase
分类号:
Q556
DOI:
10.3969/j.jssn.1000-2006.2006.03.007
文献标志码:
A
摘要:
<正>运用产业组织理论、经济学原理和企业管理理论研究了林纸一体化的动力机制模型和运行机理,并对其动因机制、产业组织机制和目标机制进行了分析。从而为促进我国林纸一体化进程,提高林纸一体化组织的生命力和竞争力提供了理论指导。
Abstract:
The endoglucanase found in Thermotoga maritirna showed extremely high thermostability and considerable potential in industrial application of enzymatic hydrolysis of cellulose. Endoglucanase (Tin) Cel12B is extracellular enzyme. Tm Cel12B did not contain a cellulose-binding domain(CBD)and lacked activity on crystalline cellulose. Tm XynA is composed of catalytic domain(CD)and cellulose-binding domain(CBD). As such, the gene of CBD from Tm XynA was fused at the earboxyl-terminus of Tm CeI12B and recombinant plasmid pET-2Ob-Cel12B-CBD was obtained. The chimera enzyme was expressed in Escherichia coli and activities were displayed on crystalline cellulose.
更新日期/Last Update: 2013-05-20