[1]张燕梅,潘惠新,张博,等.与美洲黑杨抗黑斑病基因连锁的SCAR标记的开发[J].南京林业大学学报(自然科学版),2007,31(01):015-18.[doi:10.3969/j.jssn.1000-2006.2007.01.004]
 ZHANG Yan-mei,PAN Hui-xin,ZHANG Bo,et al.Development of SCAR Markers Linked to Resistance Locus of Black Spot Disease in Populus deltoides[J].Journal of Nanjing Forestry University(Natural Science Edition),2007,31(01):015-18.[doi:10.3969/j.jssn.1000-2006.2007.01.004]
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与美洲黑杨抗黑斑病基因连锁的SCAR标记的开发
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《南京林业大学学报(自然科学版)》[ISSN:1000-2006/CN:32-1161/S]

卷:
31
期数:
2007年01期
页码:
015-18
栏目:
研究论文
出版日期:
2007-01-20

文章信息/Info

Title:
Development of SCAR Markers Linked to Resistance Locus of Black Spot Disease in Populus deltoides
文章编号:
1000-2006(2007)01-0015-04
作者:
张燕梅潘惠新张博王明庥黄敏仁*
国家林业局、江苏省林木遗传和基因工程重点实验室南京林业大学, 江苏 南京 210037
Author(s):
ZHANG Yan-mei PAN Hui-xin ZHANG Bo WANG Ming-xiu HUANG Min-ren*
The Key Laboratory of Forest Genetics and Gene Engineering of the State Forestry Administration and Jiangsu Province Nanjing Forestry University, Nanjing 210037, China
关键词:
杨树黑斑病随机扩增多态性DNA序列特异性扩增区域
Keywords:
Poplar black spot disease Random Amplified Polymorphic DNA (RAPD) Sequence-characterized Amplified Regions (SCAR)
分类号:
S722;Q813
DOI:
10.3969/j.jssn.1000-2006.2007.01.004
文献标志码:
A
摘要:
<正>以1年生金丝小枣嫁接苗为试材,研究硅对盆栽苗在盐胁迫下各胱甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)和过氧化物酶(POD)活性的影响。结果表明:在盐浓度(NaCl质量分数)小于0.4%时,SOD和GSH-PX的活性随着土壤含盐量的增加而增加。POD活性在NaCl质量分数为0.25%的盐胁迫下升高,在NaCI质量分数为0.4%的盐胁迫时下降;盐胁迫下加硅可以显著提高金丝小枣SOD、POD和GSH-Px的活性且差异显著。笔者认为盐胁迫下给土壤施硅可以缓解盐分对金丝小枣的毒害作用。
Abstract:
RAPD markers OPAI17-1550 and OPAI13-900, linked to anti-black-spot disease gene locus, were successfully converted into SCAR markers named SAI17-1570 and SAI13-292 respectively by sequencing and designing of primers. SAI17-1570 and SAI13-292 were validated in the resistant、 susceptible pools and F1 population. The SAI17-1570 had the same amplification with templates of the positive OPAI17-1550 F1, population and resistant pool, did not have amplification with templates of the negative OPAI17-1550 F1 population and susceptible pool. The SAI13-292 had one band with templates of the negative OPAI13-900 F1 population and susceptible pool, but two bands with templates of the positive OPAI13-900 F1 population and resistant pool. The two SCAR markers could be of great importance for clone selection on seedling stage.

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 GAO Jiang-yong,YANG Xiao-jun,LIN Xiao-jia,et al.A Study on the Genetic Relationships of Anoplohora with RAPD[J].Journal of Nanjing Forestry University(Natural Science Edition),2007,31(01):128.[doi:10.3969/j.jssn.1000-2006.2007.01.030]
[2]韩正敏,尹佟明.杨树过氧化物酶活性、气孔密度和大小与黑斑病抗性的关系[J].南京林业大学学报(自然科学版),1998,22(04):091.[doi:10.3969/j.jssn.1000-2006.1998.04.020]
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更新日期/Last Update: 2013-05-20